Evaluation of post‐colostrum ingestion changes in the protein composition of peripheral blood of newborn piglets: A pilot study
Evaluation of post‐colostrum ingestion changes in the protein composition of peripheral blood of newborn piglets: A pilot study
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评估初乳摄入后新生仔猪外周血蛋白质组成的变化：一项初步研究
DOI：
10.1111/asj.13783
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发表时间：
2022
影响因子：
2
通讯作者：
Inoue Ryo
中科院分区：
文献类型：
--
作者：
Ito Ken R.;Kawasaki Kiyonori;Miura Hiroto;Tsukahara Takamitsu;Inoue Ryo
在新生仔猪中，初乳蛋白被部分吸收并转移到血液循环中，这表明初乳摄入改变了血液中的蛋白质组成。在这里，我们进行了一项初步研究，以估计仔猪血液中蛋白质组成的变化。通过鸟枪蛋白质组学分析了仔猪摄入初乳前后（PreC和PostC）采集的血浆。还分析了初乳中的蛋白质。我们在PreC和PostC血浆中分别鉴定了393和427种蛋白，以及596种初乳蛋白。虽然在PostC中鉴定了202种独特的蛋白质，但PreC和PostC共有225种蛋白质。相比之下，与PreC相比，PostC中有54种蛋白质的emPAI值增加>2倍。值得注意的是，使用从单独实验收集的血浆样品，PostC中生长分化因子8和触珠蛋白的浓度高于PreC，这通过ELISA验证。PostC中大约60%的独特鉴定或高度浓缩的蛋白质也存在于初乳中，这些蛋白质可能至少部分地从初乳转移。本研究表明，新生仔猪血浆的蛋白质组成在初乳摄入后发生了显著变化，部分原因是初乳蛋白质的转移。
Putatively, colostral proteins are partly absorbed and transferred to blood circulation in newborn piglets, which suggests that colostrum ingestion alters the protein composition of their blood. Here, we conducted a pilot study to estimate the changes in the protein composition of piglet blood. Plasma collected from piglets pre‐ and post‐ingestion of colostrum (PreC and PostC) was analyzed by shotgun proteomics. Proteins in colostrum were also analyzed. We identified 393 and 427 proteins in PreC and PostC plasma, respectively, and 596 colostral proteins. Whereas 202 unique proteins were identified in PostC, PreC and PostC commonly shared 225 proteins. By contrast, when compared with PreC, 54 proteins in PostC had their emPAI values increased >2‐fold. Notably, using plasma samples collected from a separate experiment, the concentrations of growth differentiation factor 8 and haptoglobin were higher in PostC than in PreC, which was validated by ELISA. Approximately 60% of the uniquely identified or highly concentrated proteins in PostC were also found in colostrum, which were likely, at least partly, transferred from colostrum. The present study demonstrated that the protein composition of plasma of newborn piglets drastically changed post‐colostrum ingestion, partly due to transfer of colostral proteins.