Lethality of shock pressures to a marine Vibrio sp. isolated from a ship's ballast water.
Lethality of shock pressures to a marine Vibrio sp. isolated from a ship's ballast water.
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DOI：
10.4265/bio.11.159
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发表时间：
2006-12
影响因子：
1.2
通讯作者：
H. Mimura;A. Abe;Ryo Katakura;H. Kawasaki;Kazutoshi Yoshida;H. Ishida
中科院分区：
文献类型：
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作者：
H. Mimura;A. Abe;Ryo Katakura;H. Kawasaki;Kazutoshi Yoshida;H. Ishida
冲击压力处理对悬浮弧菌的致死作用。对细胞进行检测。冲击压力对弧菌的致死性。随着细胞悬浮液中产生的最大冲击压力值的增加，细胞也随之增加。当该值在114 Mpa左右时，集落形成细胞总数从10(8.5+/-0.1)个克隆形成单位(CFU)减少到10(3.3)-10(3.4)cfu/ml，克隆形成能力在最大值282 Mpa时完全丧失。几乎所有的细胞在冲击压力下都能存活，在2%抗坏血酸钠(VitC-Na)存在的情况下，最大值约为189 Mpa，而在没有VitC-Na的情况下，集落形成细胞总数减少到10(1.6)-10(2.1)cfu/ml。然而，存活的细胞对0.8%的胆酸钠，一种强大的洗涤剂表现出敏感性。当细胞暴露在致命的冲击压力下时，大约11%的细胞相关蛋白已经泄漏出来，包括在没有维生素C-Na的情况下的最大值约290兆帕。这些结果表明，细胞悬浮液中产生的自由基可能与弧菌菌落形成能力的丧失密切相关。细胞。外膜结构的损坏似乎也是由于暴露在冲击压力下造成的。
The lethal effects of shock pressure treatment on suspended Vibrio sp. cells were examined. Lethality of shock pressures to the Vibrio sp. cells increased with the increase in the values of maximum shock pressures generated in the cell suspension. When the value was around 114 MPa, the total number of colony-forming cells was reduced from 10(8.5+/-0.1) colony-forming units (CFU) to 10(3.3) - 10(3.4) CFU/ml, and complete loss of colony-forming ability was seen at the maximum value of 282 MPa. Almost all the cells could survive after the exposure to shock pressures including the maximum value of around 189 MPa in the presence of 2% sodium ascorbate (VitC-Na), whereas the total number of colony-forming cells was reduced to 10(1.6)-10(2.1) CFU/ml in the absence of VitC-Na. The surviving cells, however, showed sensitivity to 0.8% sodium cholate, a strong detergent. About 11% of cell-associated proteins had leaked out when the cells were exposed to lethal shock pressures including the maximum value of around 290 MPa in the absence of VitC-Na. These results indicate that the radicals generated in the cell suspension may be closely related to the loss of colony-forming ability of the Vibrio sp. cells. Damage to the outer membrane structure also seems to have occurred by the exposure to shock pressures.