Extension of dynamic range in Fourier transform ion cyclotron resonance mass spectrometry via stored waveform inverse Fourier transform excitation.
Extension of dynamic range in Fourier transform ion cyclotron resonance mass spectrometry via stored waveform inverse Fourier transform excitation.
复制标题
通过存储波形逆傅里叶变换激励扩展傅里叶变换离子回旋共振质谱的动态范围。
DOI：
10.1021/ac00127a009
复制
发表时间：
1986
影响因子：
7.4
通讯作者：
Marshall,AG
中科院分区：
文献类型：
--
作者：
Wang,TC;Ricca,TL;Marshall,AG
在傅里叶变换离子回旋共振（FT/ICR）质谱中相对有限的动态范围可以通过选择性喷射最丰富的离子，然后正常激发和检测剩余离子来扩展。不幸的是，扫频喷射不能提供足够的质量选择性。在本文中，我们提出存储波形逆傅立叶变换（SWIFT）激励，用于喷射高于指定强度阈值的所有离子。 SWIFT技术实现了同时快速多离子喷射，具有高质量选择性，因此可以随后以增强的信噪比和增强的质量分辨率来检测不太丰富的离子。 SWIFT 多离子喷射的理论优势在全氟三丁胺的 FT/ICR 质谱中通过实验证明了低丰度（例如碳 13 同位素）离子。傅里叶变换离子回旋共振 (FT/ICR) 质谱 (1) 提供了几个最近经过审查 (2-7) 的分析有用特征：超高质量分辨率 (> 1000000 at m/z< 200）；带 FT/ICR 检测的气相色谱精确质量测量；由于源压力比其他质谱仪低 1000 倍，因此有助于检测低挥发性样品；多功能离子源（电子轰击（El）、自化学电离（self-CI）、激光解吸（LD）、二次电离和快原子轰击（FAB））；用于研究离子/分子反应动力学、平衡和能量学的捕获离子能力；具有单个样品室和质量分析仪的质谱/质谱 (MS/MS)。然而，与其他质谱仪 (> 106:1) 相比，FT/ICR 质谱目前的主要限制是其相对较小的动态范围 (约 103:1)。在 ICR 信号强度的下限 (8) 下，检测器噪声限制了给出可观察信号所需的最小离子数量
The relatively limited dynamic range In a Fourier transform Ion cyclotron resonance(FT/ICR) mass spectrum can be ex-tended by selective ejection of the most abundant Ions, followed by normal excitation and detection of the remaining Ions. Unfortunately, frequency-sweep ejection does not pro-vide sufficient mass selectivity. In this paper, we propose stored waveform Inverse Fourier transform (SWIFT) excitation for ejection of all Ions above a specified intensity threshold. The SWIFT technique accomplishes simultaneous rapid multlple-lon ejection, with high mass selectivity, so that the less abundant ions can subsequently be detected with enhanced slgnal-to-noise ratio and enhanced mass resolution. The theoretical advantages of SWIFT multlple-lon ejection are demonstrated experimentally for low-abundance (eg, car-bon-13 isotope) Ions In the FT/ICR mass spectrum of per-fluorotributylamlne.Fourier transform ion cyclotron resonance(FT/ICR) mass spectrometry (1) offers several analytically useful features which have recently been reviewed (2-7): ultrahigh mass resolution (> 1000000 at m/z< 200); accurate mass mea-surement for gas chromatography with FT/ICR detection; facilitated detection of low-volatility samples due to 1000 times lower source pressure than in other mass spectrometers; versatile ion sources(electron impact (El), self chemical ionization (self-CI), laser desorption (LD), secondary ioniza-tion, and fast atom bombardment (FAB)); trapped-ion ca-pability for study of ion/molecule reaction kinetics, equilibria, and energetics; mass spectrometry/mass spectrometry (MS/MS) with a single sample chamber and mass analyzer. However, a major current limitation of FT/ICR mass spectrometry is its relatively small dynamic range (ca. 103: 1) compared to other mass spectrometers (> 106: 1). At the lower limit of ICR signal strength (8), detector noise limits the minimum number of ions required to give an observable signal