3D anatomy of autonomic innervations in immune organs of a non-human primate and the human
3D anatomy of autonomic innervations in immune organs of a non-human primate and the human
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DOI：
10.1016/j.fmre.2022.03.002
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发表时间：
2023-03-01
影响因子：
6.2
通讯作者：
Yang, Jing
中科院分区：
文献类型：
--
作者：
Cao, Ying;Liu, Tingting;Yang, Jing
对免疫器官的直接神经输入已经观察到几十年了，它们在神经免疫调节中的作用越来越受到重视。然而，目前关于这种神经结构的知识，特别是灵长类免疫器官中的神经结构，仍然不完整。在这项研究中，我们首次对恒河猴和人类免疫器官中自主神经(即交感神经和副交感神经)的三维解剖进行了全面的评估。借助于先进的全组织免疫标记和光片荧光成像技术，我们揭示了成年猴和人脾实质中密集组织的交感神经构筑。另一方面，在淋巴结、Peyer氏结或胸腺内只观察到少量的交感神经输入。相反，在这些被检查的免疫器官的实质中有最小的副交感神经支配。这项工作记录了非人灵长类动物和人类不同免疫器官中自主神经支配的独特模式，为未来该领域的神经免疫调节研究提供了必要的参考。
Direct neural inputs to immune organs have been observed for decades, with their functions in neuroimmune regulation being increasingly appreciated. However, the current knowledge of such neural structures, particularly those in primate immune organs, remains incomplete. In this study, we comprehensively assessed the 3D anatomy of autonomic (i.e., sympathetic and parasympathetic) innervations in the immune organs of the rhesus macaque monkey and the human for the first time. Aided with the advanced technique of whole-tissue immunolabeling and lightsheet fluorescence imaging, we revealed the densely organized sympathetic architecture in the parenchyma of the adult monkey and human spleens. On the other hand, only sparse, if any, sympathetic inputs were observed inside the lymph nodes, Peyer's patches, or thymus. In contrast, there were minimal parasympathetic innervations in the parenchyma of these examined immune organs. Together, this work has documented the unique patterns of autonomic innervations in different immune organs of a non-human primate and the human, serving as an essential reference for future research on neuroimmune regulation in the field.