Evidence for Horizontal and Vertical Transmission of Mtr-Mediated Extracellular Electron Transfer among the Bacteria.
Evidence for Horizontal and Vertical Transmission of Mtr-Mediated Extracellular Electron Transfer among the Bacteria.
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DOI：
10.1128/mbio.02904-21
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发表时间：
2021-02-22
期刊：
影响因子：
6.4
通讯作者：
Girguis PR
中科院分区：
文献类型：
--
作者：
Baker IR;Conley BE;Gralnick JA;Girguis PR
一些细菌和古细菌已经进化出利用细胞外电子供体和受体进行能量代谢的方法，这种现象广泛地称为细胞外电子转移（EET）。一种这样的EET机制是跨膜电子导管MtrCAB，其已被证明将源自代谢底物的电子转移到细胞外的电子受体，如Fe（III）和Mn（IV）氧化物。虽然大多数研究MtrCAB介导的EET已经进行了希瓦氏菌oneidensis MR-1，弧菌和气单胞菌属物种的最近的调查表明，在希瓦氏菌中支持MtrCAB的供电子蛋白并不像以前认为的那样具有代表性。这就引出了一个问题，即MtrCAB介导的EET的能力有多广泛，它在不同谱系中产生的变化，以及这些谱系今天在哪里持续存在。在这里，我们采用了系统发育和比较基因组学的方法，以确定跨所有领域的生活的MtrCAB系统。我们在来自广泛环境的许多不同细菌的基因组中发现了mtrCAB，并且其中的模式强烈表明mtrCAB通过水平和随后的垂直传播分布，并且某些情况下表明其核心和附属组分的下游模块化多样化。我们的数据指出了一个新兴的进化故事，关于金属氧化和还原代谢，表明EET的这种能力与它们驱动的生物分类群和生物地球化学循环的多样性具有广泛的相关性，并为进一步研究奠定了基础，以阐明这种机制可能与地球的氧化还原景观共同进化。
Some bacteria and archaea have evolved the means to use extracellular electron donors and acceptors for energy metabolism, a phenomenon broadly known as extracellular electron transfer (EET). One such EET mechanism is the transmembrane electron conduit MtrCAB, which has been shown to transfer electrons derived from metabolic substrates to electron acceptors, like Fe(III) and Mn(IV) oxides, outside the cell. Although most studies of MtrCAB-mediated EET have been conducted in Shewanella oneidensis MR-1, recent investigations in Vibrio and Aeromonas species have revealed that the electron-donating proteins that support MtrCAB in Shewanella are not as representative as previously thought. This begs the question of how widespread the capacity for MtrCAB-mediated EET is, the changes it has accrued in different lineages, and where these lineages persist today. Here, we employed a phylogenetic and comparative genomics approach to identify the MtrCAB system across all domains of life. We found mtrCAB in the genomes of numerous diverse Bacteria from a wide range of environments, and the patterns therein strongly suggest that mtrCAB was distributed through both horizontal and subsequent vertical transmission, and with some cases indicating downstream modular diversification of both its core and accessory components. Our data point to an emerging evolutionary story about metal-oxidizing and -reducing metabolism, demonstrates that this capacity for EET has broad relevance to a diversity of taxa and the biogeochemical cycles they drive, and lays the foundation for further studies to shed light on how this mechanism may have coevolved with Earth’s redox landscape.
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