Interactions of corneal epithelial cells and surfaces modified with cell adhesion peptide combinations
Interactions of corneal epithelial cells and surfaces modified with cell adhesion peptide combinations
复制标题
DOI：
10.1163/156856202320253956
复制
发表时间：
2002-01-01
影响因子：
3.6
通讯作者：
Sheardown, H
中科院分区：
文献类型：
--
作者：
Aucoin, L;Griffith, CM;Sheardown, H
为了促进角膜上皮细胞与聚二甲基硅氧烷(PDMS)基质的黏附，最终开发出一种人工角膜假体，通过细胞黏附和层粘连蛋白和纤维连接蛋白产生的协同多肽的共价黏附来修饰PDMS表面。所研究的多肽包括来自层粘连蛋白的YIGSR及其协效肽PDSGR，以及纤维连接蛋白衍生的RGDS和PHSRN。用实验设计确定的多肽组合对表面进行修饰。用125-I标记的酪氨酸类似物测得的多肽表面密度约为pmol/cm(2)。表面密度与反应溶液中的多肽浓度成线性函数关系，不同的多肽所考察的表面密度是不同的。在所有溶液组分中，GYRGDS的表面密度最低，而GYPDSGR的表面密度最高。这些结果为表面修饰了多种多肽提供了证据。水接触角和XPS结果为不同表面的化学成分的差异提供了额外的证据。人角膜上皮细胞与修饰表面的粘附性有显著差异。对实验黏附结果的统计分析表明，YIGSR、RGDS和PHSRN的溶液浓度以及YIGSR和PDSGR的相互作用对细胞相互作用有显著影响。多肽修饰比单一多肽修饰具有更强的粘附性。用控制肽PPSRN代替PHSRN进行表面修饰，几乎在所有情况下都能降低细胞粘附性。这些结果表明，用适当的细胞粘附肽和协同多肽的组合进行表面修饰可能会改善细胞表面的相互作用。
In order to facilitate the adhesion of corneal epithelial cells to a poly dimethyl siloxane (PDMS) substrate ultimately for the development of a synthetic keratoprosthesis, PDMS surfaces were modified by covalent attachment of combinations of cell adhesion and synergistic peptides derived from laminin and fibronectin. Peptides studied included YIGSR and its synergistic peptide PDSGR from laminin and the fibronectin derived RGDS and PHSRN. Surfaces were modified with combinations of peptides determined by an experimental design. Peptide surface densities, measured using 125-I labeled tyrosine containing analogs, were on the order of pmol/cm(2). Surface density varied as a linear function of peptide concentration in the reaction solution, and was different for the different peptides examined. The lowest surface density at all solution fractions was obtained with GYRGDS, while the highest density was consistently obtained with GYPDSGR. These results provide evidence that the surfaces were modified with multiple peptides. Water contact angles and XPS results provided additional evidence for differences in the chemical composition of the various surfaces. Significant differences in the adhesion of human corneal epithelial cells to the modified surfaces were noted. Statistical analysis of the experimental adhesion results suggested that solution concentration YIGSR, RGDS, and PHSRN as well as the interaction effect of YIGSR and PDSGR had a significant effect on cell interactions. Modification with multiple peptides resulted in greater adhesion than modification with single peptides only. Surface modification with a control peptide PPSRN in place of PHSRN resulted in a decrease in cell adhesion in virtually all cases. These results suggest that surface modification with appropriate combinations of cell adhesion peptides and synergistic peptides may result in improved cell surface interactions.