DNA barcodes reveal cryptic host-specificity within the presumed polyphagous members of a genus of parasitoid flies (Diptera: Tachinidae)
DNA barcodes reveal cryptic host-specificity within the presumed polyphagous members of a genus of parasitoid flies (Diptera: Tachinidae)
复制标题
DOI：
10.1073/pnas.0511318103
复制
发表时间：
2006-03-07
影响因子：
11.1
通讯作者：
Hebert, PDN
中科院分区：
文献类型：
--
作者：
Smith, MA;Woodley, NE;Hebert, PDN
昆虫寄生蜂是全球生物多样性的重要组成部分，影响着寄主的种群动态。然而，昆虫拟寄生蜂的鉴定往往是困难的，他们被怀疑含有许多神秘的物种。在这里，我们问细胞色素c氧化酶I DNA条形码是否可以作为一种工具，已饲养的毛毛虫（鳞翅目）在瓜纳卡斯特保护区（ACG），西北部哥斯达黎加的20形态种Belvosia寄生蝇（双翅目：寄蝇科）的物种鉴定和发现。条形码不仅区分所有17个高度宿主特异性形态种的ACG Belvosia，但它也提高了物种计数到32揭示，每三个通才物种实际上是阵列的高度宿主特异性的神秘物种。我们还确定了可能的杂交Belvosia之间通过使用可变的内部转录间隔区1核rDNA序列作为遗传协变量，除了与生态信息覆盖条形码簇的策略。如果普遍，这些结果将增加全球物种丰富度的估计，并意味着热带保护和宿主-寄生虫相互作用可能比预期的更复杂。
Insect parasitoids are a major component of global biodiversity and affect the population dynamics of their hosts. However, identification of insect parasitoids is often difficult, and they are suspected to contain many cryptic species. Here, we ask whether the cytochrome c oxidase I DNA barcode could function as a tool for species identification and discovery for the 20 morphospecies of Belvosia parasitoid flies (Diptera: Tachinidae) that have been reared from caterpillars (Lepidoptera) in Area de Conservacion Guanacaste (ACG), northwestern Costa Rica. Barcoding not only discriminates among all 17 highly host-specific morphospecies of ACG Belvosia, but it also raises the species count to 32 by revealing that each of the three generalist species are actually arrays of highly host-specific cryptic species. We also identified likely hybridization among Belvosia by using a variable internal transcribed spacer region 1 nuclear rDNA sequence as a genetic covariate in addition to the strategy of overlaying barcode clusters with ecological information. If general, these results will increase estimates of global species richness and imply that tropical conservation and host-parasite interactions may be more complex than expected.