Sequential Changes in the Host Gut Microbiota During Infection With the Intestinal Parasitic Nematode Strongyloides venezuelensis
Sequential Changes in the Host Gut Microbiota During Infection With the Intestinal Parasitic Nematode Strongyloides venezuelensis
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DOI：
10.3389/fcimb.2019.00217
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发表时间：
2019-06-25
影响因子：
5.7
通讯作者：
Kikuchi, Taisei
中科院分区：
文献类型：
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作者：
Afrin, Tanzila;Murase, Kazunori;Kikuchi, Taisei
土壤传播蠕虫（STH）是医学上重要的寄生虫，感染1。全球50亿人，造成巨大的疾病负担。这些寄生虫感染其宿主的胃肠道（GIT），在那里它们与宿主肠道细菌植物群共存并相互作用，导致寄生虫、微生物群和宿主生物体的共同进化。然而，人们对这些相互作用如何随着感染的进展而随时间变化知之甚少。类圆线虫病是由粪类圆线虫引起的人类寄生虫病，感染3000万至1亿人。在这项研究中，我们使用密切相关的啮齿类寄生虫委内瑞拉圆线虫和小鼠作为胃肠道寄生虫感染的模型。我们进行了一项时间过程实验，以检查从感染开始到寄生虫清除的粪便微生物群的变化。我们发现，宿主肠道微生物群中的细菌类群随着感染的进展而发生显著变化，拟杆菌属和拟杆菌属Arthromitus增加，普雷沃氏菌属和Rikenellaceae减少。然而，在寄生虫从宿主清除后，微生物群恢复到感染前状态，表明这些扰动是可逆的。微阵列分析显示，这种微生物群的转变可能与宿主的免疫反应相对应。这些发现使我们深入了解寄生虫感染期间宿主肠道中寄生虫-微生物群相互作用的动态。
Soil-transmitted helminths (STHs) are medically important parasites that infect 1. 5 billion humans globally, causing a substantial disease burden. These parasites infect the gastrointestinal tract (GIT) of their host where they co-exist and interact with the host gut bacterial flora, leading to the coevolution of the parasites, microbiota, and host organisms. However, little is known about how these interactions change through time with the progression of infection. Strongyloidiasis is a human parasitic disease caused by the nematode Strongyloides stercoralis infecting 30-100 million people. In this study, we used a closely related rodent parasite Strongyloides venezuelensis and mice as a model of gastrointestinal parasite infection. We conducted a time-course experiment to examine changes in the fecal microbiota from the start of infection to parasite clearance. We found that bacterial taxa in the host intestinal microbiota changed significantly as the infection progressed, with an increase in the genera Bacteroides and Candidatus Arthromitus, and a decrease in Prevotella and Rikenellaceae. However, the microbiota recovered to the pre-infective state after parasite clearance from the host, suggesting that these perturbations are reversible. Microarray analysis revealed that this microbiota transition is likely to correspond with the host immune response. These findings give us an insight into the dynamics of parasite-microbiota interactions in the host gut during parasite infection.