Production of polyclonal antibodies to Batrachochytrium dendrobatidis and their use in an immunoperoxidase test for chytridiomycosis in amphibians
Production of polyclonal antibodies to Batrachochytrium dendrobatidis and their use in an immunoperoxidase test for chytridiomycosis in amphibians
复制标题
DOI：
10.3354/dao048213
复制
发表时间：
2002-04-05
影响因子：
1.4
通讯作者：
Longcore, JE
中科院分区：
文献类型：
--
作者：
Berger, L;Hyatt, AD;Longcore, JE
生产多克隆抗体用于诊断两栖动物的壶菌病。用在弗氏完全佐剂或三重佐剂中的均质化的树状壶菌完整培养物接种两只绵羊和四只兔子。在间接免疫过氧化物酶测试中，所有动物的抗血清均与所有阶段的树突芽孢杆菌发生强烈反应，并对细胞壁、细胞质、假根和游动孢子进行染色。显着的交叉反应性 仅发生在壶菌门中的某些真菌中，并且除了 B. dendrobatidis 之外，该门中没有其他成员感染青蛙。当与树状芽孢杆菌形态和感染部位的识别相结合时，免疫过氧化物酶染色是一种有用的筛选试验。
Polyclonal antibodies were produced for diagnosing chytridiomycosis in amphibians. Two sheep and 4 rabbits were inoculated with homogenized whole culture of Batrachochytrium dendrobatidis in Freund's complete adjuvant or triple adjuvant. Antisera from all animals reacted strongly with all stages of B. dendrobatidis and stained the walls, cytoplasm, rhizoids and zoospores in an indirect immunoperoxidase test. Significant cross-reactivity Occurred only with some fungi in the Chytridiomycota, and there are no members of this phylum besides B. dendrobatidis that infect frogs. The immunoperoxidase stain is a useful screening test when combined with recognition of the morphology and infection site of B. dendrobatidis.