Assessment of oxidative stress biomarkers in exhaled breath condensate and blood of dairy heifer calves from birth to weaning
Assessment of oxidative stress biomarkers in exhaled breath condensate and blood of dairy heifer calves from birth to weaning
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DOI：
10.1016/j.tvjl.2014.10.025
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发表时间：
2014-12-01
影响因子：
2.2
通讯作者：
Celi, Pietro
中科院分区：
文献类型：
--
作者：
Ranade, Rujuta;Talukder, Saranika;Celi, Pietro
新生小牛中氧化剂和抗氧化剂之间的平衡可能会受损，但关于健康小牛中氧化生物标志物的生理变化的信息有限。本研究的目的是测量氧化应激（OS）的生物标志物在犊牛从出生到断奶。研究中使用了18头健康雌性荷斯坦-弗里斯兰小牛。在出生当天（第0周）以及第6、12和18周采集血液和呼出气冷凝液（EBC）样本。对新鲜血液测定活性氧代谢产物（ROMs）和生物抗氧化潜力（BAP），并将OS程度表示为氧化应激指数（OSI），其中OSI = ROMs/BAP x 100。还测量了抗氧化屏障（OXY）、巯基抗氧化屏障（SH）、晚期氧化蛋白产物（AOPP）、白蛋白和非酯化脂肪酸（NEFA）的血浆浓度，以及EBC中的过氧化氢（H2 O2）浓度。试验期间ROM的血浆浓度相对稳定; AOPP浓度逐渐降低，在第18周达到最低值。从出生到断奶，白蛋白、SH和OXY浓度逐渐增加。血浆中NEFA和EBC中H2 O2的浓度相对稳定，但在第18周达到峰值。结果表明，抗氧化防御不仅随着时间的推移，在新生小牛的血浆中，但它也与蛋白质氧化过程。此外，数据支持使用EBC H2 O2作为评估小牛呼吸OS的新型生物标志物。(C)2014爱思唯尔有限公司版权所有。
The balance between oxidants and antioxidants may be impaired in neonatal calves but only limited information is available on physiological changes in oxidative biomarkers in healthy calves. The aim of this study was to measure biomarkers of oxidative stress (OS) in calves from birth to weaning. Eighteen, healthy, female Holstein-Friesian calves were used in the study. Blood and exhaled breath condensate (EBC) samples were taken on the day of birth (Week 0) and then at Weeks 6, 12 and 18. Reactive oxygen metabolites (ROMs) and biological antioxidant potential (BAP) were determined on fresh blood and the degree of OS was expressed as an oxidative stress index (OSI), where OSI = ROMs/BAP x 100. Plasma concentrations of antioxidant barrier (OXY), thiol antioxidant barrier (SH), advanced oxidation protein products (AOPP), albumin, and non-esterified fatty acids (NEFA) were also measured, as was hydrogen peroxide (H2O2) concentration in EBC.Plasma concentrations of ROMs were relatively stable during the trial; AOPP concentration progressively decreased reaching its lowest values on Week 18. Albumin, SH and OXY concentrations progressively increased from birth to weaning. Concentrations of NEFA in plasma and H2O2 in EBC were relatively stable but peaked in Week 18. The results indicate that antioxidative defence not only increases with time in the plasma of new-born calves but also that it is related to protein oxidation processes. Furthermore, the data support the use EBC H2O2 as a novel biomarker to assess respiratory OS in calves. (C) 2014 Elsevier Ltd. All rights reserved.