Premeiotic fetal murine germ cells cultured in vitro form typical oocyte-like cells but do not progress through meiosis
Premeiotic fetal murine germ cells cultured in vitro form typical oocyte-like cells but do not progress through meiosis
复制标题
体外培养的减数分裂前胎儿小鼠生殖细胞形成典型的卵母细胞样细胞，但不会通过减数分裂进行进展
DOI：
10.1016/j.theriogenology.2009.02.021
复制
发表时间：
2009-07-15
期刊：
影响因子：
2.8
通讯作者：
Shen, W.
中科院分区：
文献类型：
--
作者：
Dong, H. S.;Li, L.;Shen, W.
建立了一种方便的小鼠胚胎减数前生殖细胞体外发育为卵母细胞的方法。取交媾后12.5 d的小鼠胚胎卵巢，用器官生长培养基进行体外器官培养，测定卵母细胞形成的发育潜力。培养28 d后，雌性减数分裂前生殖细胞发育为卵母细胞，平均（+/- SD）直径为73.3 +/- 7.7 μ m，体外卵泡颗粒细胞数量少于体内卵泡（32 +/- 2.6 vs. 142 +/- 9.5, P < 0.01），卵巢中Cx37和Cx43 mRNA表达量少于体内卵泡（P < 0.01）。从体外培养的卵巢中分离出减数分裂第一阶段的卵母细胞或经激素处理的卵母细胞。在最终浓度为1 μ M的冈田酸作用下，来自于减数分裂前胎儿雌性生殖细胞的卵母细胞能够发生生发囊泡破裂，但不能完成第一次减数分裂。体外培养卵母细胞细胞内GSH含量低于体内成熟卵母细胞（P < 0.01）。综上所述，早在12.5 dpc的小鼠胚胎中获得的减数分裂前生殖细胞能够分化为萌发囊泡期卵母细胞，但在体外不能完成减数分裂I。爱思唯尔公司2009年版权所有版权所有。
A convenient method for fetal murine premeiotic germ cells to develop into oocytes in vitro has been established. Fetal ovaries from mice, collected 12.5 d postcoitus (dpc), were organ-cultured in vitro using a medium for organ growth, and the developmental potential regarding oocyte, formation was determined. After 28 d of culture, premeiotic female germ cells developed into oocytes with a mean (+/- SD) diameter of 73.3 +/- 7.7 mu m. However, follicles developed in vitro versus ill vivo had fewer granulosa cells (32 +/- 2.6 vs. 142 +/- 9.5, respectively; P < 0.01), and the ovaries had less mRNA for Cx37 and Cx43 (P < 0.01). Oocytes ill the first meiotic division phase were isolated from cultured ovaries or after hormone treatments. After exposure to okadaic acid at a final concentration of 1 mu M, oocytes derived from premeiotic fetal female germ cells were able to undergo germinal vesicle breakdown but failed to complete the first meiotic division. Furthermore, the intracellular content of GSH in oocytes cultured in vitro was lower than that of oocytes matured in vivo (P < 0.01). In conclusion, premeiotic germ cells derived from murine fetuses as early as 12.5 dpc were able to differentiate into germinal vesicle-stage oocytes but were unable to complete meiosis I in vitro. Crown Copyright (C) 2009 Published by Elsevier Inc. All rights reserved.