Chemical heterogeneity of humic substances: characterization of size fractions obtained by hollow‐fibre ultrafiltration
Chemical heterogeneity of humic substances: characterization of size fractions obtained by hollow‐fibre ultrafiltration
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DOI：
10.1111/j.1365-2389.2000.00352.x
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发表时间：
2000-12
影响因子：
4.2
通讯作者：
I. Christl;H. Knicker;I. Kögel‐Knabner;R. Kretzschmar
中科院分区：
文献类型：
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作者：
I. Christl;H. Knicker;I. Kögel‐Knabner;R. Kretzschmar
为了研究腐殖物质的化学异质性与分子大小的关系，从瑞士北方的腐殖潜育土表层提取并纯化了富里酸和腐殖酸。开发了使用中空纤维超滤盒的分级方案，并用于获得标称分子量范围> 300 kDa、100-300 kDa、30-100 kDa和10-30 kDa的腐殖酸的四种尺寸级分。通过尺寸排阻色谱法、元素分析（C、H、N、S）以及光谱技术（包括UV-维斯、CP-MAS 13 C-NMR、FT-IR和荧光光谱）来表征富里酸和所有腐殖酸级分。
To investigate the chemical heterogeneity of humic substances in relation to molecular size, fulvic and humic acids were extracted and purified from the surface horizon of a Humic Gleysol in northern Switzerland. A fractionation scheme using hollow‐fibre ultrafiltration cartridges was developed and used to obtain four size fractions of the humic acid with nominal molecular weight ranges > 300 kDa, 100–300 kDa, 30–100 kDa, and 10–30 kDa. The fulvic acid and all humic acid fractions were characterized by size exclusion chromatography, elemental analysis (C, H, N, S), as well as spectroscopic techniques including UV‐VIS, CP‐MAS 13C‐NMR, FT‐IR, and fluorescence spectroscopy.