Persistence of maternal RNA in Engystomops embryos.
Persistence of maternal RNA in Engystomops embryos.
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Engystomops 胚胎中母体 RNA 的持久性。
DOI：
10.1002/jez.1401850310
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发表时间：
1973
期刊：
影响因子：
--
通讯作者：
E. Davidson
中科院分区：
文献类型：
--
作者：
B. Hough;P. Yancey;E. Davidson
雌性的neotropic无尾类Engystomops pustulosus注射3 H-尿苷，并产生含有RNA标记的卵子在同步卵子发生。从这些卵和从它们发育的胚胎中提取的>4S标记的RNA与Engystomops DNA的分离的重复部分杂交，并在羟基磷灰石柱上分析杂交体。大约1%的卵RNA是从该部分中包含的DNA序列转录的。标记的核糖体RNA的杂交被最小化，通过添加过量的未标记的从非洲爪蟾卵巢提取的核糖体RNA。通过与未标记的圆口蛙卵母细胞RNA的竞争实验，鉴定了蝌蚪期胚胎的标记RNA为母体转录物。结果发现，母体标记的RNA保留在胚胎中至少到蝌蚪阶段。
Females of the neotropic anuran Engystomops pustulosus were injected with 3H-uridine and produced eggs containing RNA labeled during synchronous oogenesis. The >4S, labeled RNA extracted from these eggs, and from embryos which developed from them, was hybridized with an isolated repetitive fraction of Engystomops DNA, and the hybrids were assayed on hydroxyapatite columns. About 1% of the egg RNA is transcribed from DNA sequences included in this fraction. Hybridization of labeled ribosomal RNA was minimized by the addition of excess unlabeled ribosomal RNA extracted from Xenopus ovaries. The labeled RNA of embryos up to the tadpole stage was identified as maternal transcript by competition experiments with unlabeled Engystomops oocyte RNA. It was found that maternally-labeled RNA is retained in embryos at least up to the tadpole stage.