HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF HUMAN HEMOGLOBINS - SIMULTANEOUS QUANTITATION OF FETAL AND GLYCATED HEMOGLOBINS
HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF HUMAN HEMOGLOBINS - SIMULTANEOUS QUANTITATION OF FETAL AND GLYCATED HEMOGLOBINS
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DOI：
10.1016/0378-4347(88)80065-3
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发表时间：
1988-12-29
期刊：
影响因子：
--
通讯作者：
WIELAND, H
中科院分区：
文献类型：
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作者：
BISSE, E;WIELAND, H
描述了一种高效液相色谱系统，该系统使用弱阳离子交换剂 (PolyCATA) 以及 Bis-Tris 缓冲液 (pH 6.47-7.0) 和乙酸钠梯度。对成人和新生儿的样本进行了分析，并大大改善了许多次要和主要的正常和异常血红蛋白 (Hb) 变异的清晰分离。该方法可以分离轻微的胎儿血红蛋白 (HbF) 变异，并同时定量 HbF 和糖化 HbA。 HbF 值与碱变性法获得的值相关性良好 (r=0.997)。在具有高 HbF 浓度的患者中测量的糖化血红蛋白 (HbA1c) 水平与通过生物亲和色谱法测定的总糖化血红蛋白相关 (r=0.973)。该程序对于诊断应用很有用，并提供了一种有效且灵敏的方法来检查血液样本中的血红蛋白异常。
A high-performance liquid chromatographic system, which uses a weak cation exchanger (PolyCATA) together with Bis-Tris buffer (pH 6.47-7.0) and sodium acetate gradients, is described. Samples from adults and newborns were analysed and a clean separation of many minor and major normal and abnormal haemoglobin (Hb) variants was greatly improved. The method allows the separation of minor foetal haemoglobin (HbF) variants and the simultaneous quantitation of HbF and glycated HbA. HbF values correlated well with those obtained by the alkali denaturation method (r=0.997). The glycated haemoglobin (HbAIc) levels measured in patients with high HbF concentrations correlated with the total glycated haemoglobin determined by bioaffinity chromatography (r=0.973). The procedure is useful for diagnostic applications and affords an effective and sensitive way of examining blood samples for haemoglobin abnormalities.