RAPID NON-GENOMIC ACTIONS OF PROGESTERONE STIMULATE CA2+ INFLUX AND THE ACROSOME REACTION IN HUMAN SPERM
RAPID NON-GENOMIC ACTIONS OF PROGESTERONE STIMULATE CA2+ INFLUX AND THE ACROSOME REACTION IN HUMAN SPERM
复制标题
DOI：
10.1016/0898-6568(93)90048-q
复制
发表时间：
1993-09-01
影响因子：
4.8
通讯作者：
BLACKMORE, PF
中科院分区：
文献类型：
--
作者：
BLACKMORE, PF
本文综述了近年来在人类精子中的一些发现，这些发现表明孕酮和17 α -羟孕酮能够通过非基因组细胞表面受体快速（在几秒钟内）提高[Ca2+]i并引发顶体反应（AR）。孕酮促进[Ca2+]i的短暂升高，这被细胞外La3+和Ni2+阻断，并在与EGTA螯合后去除细胞外Ca2+。一些研究表明，多胺、蛋白酶样蛋白酶和孕激素受体聚集参与孕激素诱导的Ca2+内流和AR。孕激素受体不受强效合成孕激素（如孕酮、去甲孕酮、醋酸甲孕酮、醋酸环丙孕酮）的刺激，并被基因组抗孕激素RU 486和ZK 98.299弱拮抗。镇静催眠作用的3 α羟基A环还原孕激素是GABA(A) Cl-通道的强激活剂，是Ca2+内流和AR的弱激活剂。这些数据表明，人类精子有一个细胞表面类固醇受体，不同于基因组孕酮受体和GABA(A) Cl-通道类固醇受体。
This review summarizes some recent findings in human sperm which show that progesterone and 17alpha hydroxyprogesterone are able rapidly (within seconds) to elevate [Ca2+]i and elicit the acrosome reaction (AR) via a non-genomic cell surface receptor. Progesterone promotes a transient elevation in [Ca2+]i which is blocked by extracellular La3+ and Ni2+ and removal of extracellular Ca2+ following chelation with EGTA. Some studies suggest that polyamines, trypsin-like proteases, and progesterone receptor aggregation are involved in progesterone-induced Ca2+ influx and AR. The receptor is not stimulated by the potent synthetic progestigins (e.g. promegestone, norethynodrel, megestrol acetate, cyproterone acetate) and is weakly antagonized by the genomic anti-progestins RU 486 and ZK 98.299. The sedative-hypnotic 3alpha hydroxyl A-ring reduced pregnane steroids, which are powerful activators of the GABA(A) Cl- channel, are weak activators of Ca2+ influx and the AR. These data suggest that human sperm have a cell surface steroid receptor which is unlike the genomic progesterone receptor and the GABA(A) Cl- channel steroid receptor.