The secY-rpmJ region of the spc ribosomal protein operon in Escherichia coli: structural alterations affecting secY expression.
The secY-rpmJ region of the spc ribosomal protein operon in Escherichia coli: structural alterations affecting secY expression.
复制标题
大肠杆菌中 spc 核糖体蛋白操纵子的 secY-rpmJ 区域：影响 secY 表达的结构改变。
DOI：
10.1007/bf00330934
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Ito,K
中科院分区：
文献类型：
--
作者：
Ueguchi,C;Wittekind,M;Nomura,M;Akiyama,Y;Ito,K
核糖体蛋白操纵子的一个独特特征是其远端启动子区域包含一个完整膜蛋白的基因（secY），随后是一个被称为X的开放阅读框，最近被提出编码一个新的核糖体蛋白（蛋白B）。我们现在表明，开放阅读框X确实指导了一种与B蛋白相似的具有电泳迁移性的蛋白质的合成，这支持了X可能更适合称为drpmj的建议。将质粒序列插入染色体的cy - rpmj边界，可能通过破坏信息的cy - rpmj部分的稳定，导致secy的表达减少。补体实验结果表明，正常水平的rpmjis表达不需要生长或蛋白质分泌。
A unique feature of thespcribosomal protein operon is that its region distal to the promoter contains a gene (secY) for an integral membrane protein, followed by an open reading frame termed X which has recently been proposed to encode a new ribosomal protein (protein B). We now show that the open reading frame X indeed directs the synthesis of a protein with electrophoretic mobilities similar to the B protein, and this supports the proposal that X may be more appropriately calledrpmJ. Insertion of a plasmid sequence into thesecY-rpmJboundary of the chromosome caused a reduced expression ofsecYprobably by destabilizing thesecYpart of the message. The results of complementation experiments suggested that a normal level of expression ofrpmJis not required for growth or protein secretion.