ASTROCYTES AND SCHWANN-CELLS ARE VIRUS-HOST CELLS IN THE NERVOUS-SYSTEM OF RATS WITH BORNA DISEASE
ASTROCYTES AND SCHWANN-CELLS ARE VIRUS-HOST CELLS IN THE NERVOUS-SYSTEM OF RATS WITH BORNA DISEASE
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DOI：
10.1097/00005072-198911000-00005
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发表时间：
1989-11-01
影响因子：
3.2
通讯作者：
NARAYAN, O
中科院分区：
文献类型：
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作者：
CARBONE, KM;TRAPP, BD;NARAYAN, O
博尔纳病病毒(BDV)只在成年大鼠中枢(CNS)和外周(PNS)神经系统的细胞中复制。神经系统感染与短暂、剧烈的单核脑膜脑炎和免疫介导的BDV感染神经元丢失有关。BDV抗原在神经元中的识别以及伴随而来的这些细胞的免疫特异性裂解导致了预测，即动物从脑炎中恢复后，中枢神经系统将不再携带病毒。然而，BDV的传染性和抗原性在动物的一生中持续存在。因此，似乎其他神经细胞可能是病毒复制的宿主，并为病毒提供了一个储存库。用形态学标准鉴定体内表达BDV抗原的星形胶质细胞和雪旺细胞。博尔纳病病毒(BDV)感染星形胶质细胞后，用细胞特异性抗体和BDV特异性抗体结合免疫细胞化学方法进行双重标记。对连续的1微米厚的海马体和坐骨神经冰冻切片的检查发现，有几个细胞同时表达胶质细胞和BDV抗原。从感染大鼠的雪旺细胞培养中回收感染病毒。博尔纳病病毒感染的神经胶质细胞在炎症和大规模神经元破坏期间持续存在，并在慢性病期间代表一类主要的感染细胞。
Borna disease virus (BDV) replicates only in cells in the central (CNS) and peripheral (PNS) nervous system in adult rats. Infection of the nervous system is associated with a transient, intense mononuclear meningoencephalitis and immune-mediated loss of BDV-infected neurons. The identification of BDV antigen in neurons and the accompanying immunologically-specific lysis of these cells led to the prediction that the CNS would be virus-free after the animal had recovered from encephalitis. However, BDV infectivity and antigen persist for the lifetime of the animal. It appeared, therefore, that other neural cells might be hosts for viral replication and provide a reservoir for the virus. Morphological criteria were used to identify astrocytes and Schwann cells which expressed BDV antigens in vivo. Borna disease virus (BDV) infected astrocytes were identified by double labeling tissue sections with combined cell-specific and BDV-specific antibodies in an avidin-biotin immunocytochemical assay. Examination of serial 1 micrometer-thick cryosections of hippocampus and sciatic nerve preparations revealed several cells that expressed both glial and BDV antigens. Infections virus was recovered from cultures of Schwann cells from infected rats. Borna disease virus-infected glial elements persisted beyond the period of inflammation and massive neuronal destruction, and represented a major class of infected cells during chronic disease.