Regulation of antigen presentation by acidic pH.
Regulation of antigen presentation by acidic pH.
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通过酸性pH调节抗原表现。
DOI：
10.1084/jem.171.5.1779
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发表时间：
1990-05-01
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中科院分区：
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pH对肽抗原与APC膜的功能性结合的影响通过使用酶解固定的B细胞和II类限制性T细胞杂交瘤来评估抗原/MHC复合物的形成来研究。结果表明，与pH 7.3相比，pH 5.0时功能肽结合的速率和程度显著增加。用pH 5.0缓冲液预处理固定的APC后，保留了结合的pH依赖性，表明pH对肽与APC膜的相互作用有直接影响。通过使用几种肽和I-Ad-和I-Ed-限制性T细胞获得了类似的结果，表明pH在调节功能性抗原/II类MHC复合物的形成中可能具有普遍的重要性。
The effect of pH on functional association of peptide antigens with APC membranes was investigated by using aldehyde-fixed B cells and class II- restricted T cell hybridomas to assess antigen/MHC complex formation. The results indicated that the rate and extent of functional peptide binding was markedly increased at pH 5.0 as compared with pH 7.3. The pH dependence of binding was preserved after pretreatment of fixed APC with pH 5.0 buffer, suggesting that pH had a direct effect on the interaction of peptide with the APC membrane. Similar results were obtained by using several peptides and I-Ad- and I-Ed-restricted T cells, indicating that pH may be of general importance in regulating the formation of functional antigen/class II MHC complexes.