Review and perspectives on microfluidic flow cytometers
Review and perspectives on microfluidic flow cytometers
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DOI：
10.1016/j.snb.2018.03.091
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发表时间：
2018-08
期刊：
影响因子：
--
通讯作者：
Ruey-Jen Yang;Lung-Ming Fu;H. Hou
中科院分区：
文献类型：
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作者：
Ruey-Jen Yang;Lung-Ming Fu;H. Hou
现代微流细胞仪是一种先进的仪器，能够同时测量单个细胞在悬浮状态下流经测量设备时的多个物理特性。细胞仪是高速采集和分析大型单细胞群体的首选工具。然而，传统设备缺乏提供细胞内空间信息的能力。在过去的几十年里，已经提出了各种能够结合细胞/颗粒检测和获取二维或三维空间信息的流式细胞仪系统。然而，这些设备面临着许多缺点，因此，基于电阻抗、光学检测和图像分析方法开发更复杂的微流控细胞仪的问题在文献中受到了极大的关注。本文综述了近十年来微流控细胞术领域取得的一些重要进展。该综述特别侧重于最近提出的关于强化微流控聚焦技术和检测/分析方法的建议。总体而言，这篇综述为新用户和现有用户提供了对微流式细胞仪技术领域的有用洞察。
Modern microflow cytometers are sophisticated instruments capable of measuring multiple physical characteristics of a single cell simultaneously as the cells flow in suspension through a measuring device. Cytometers are the tool of choice for the high-speed acquisition and analysis of large single cell populations. However, traditional devices lack the ability to provide intracellular spatial information. In the past few decades, various flow cytometer systems with the ability to combine cell/particle detection and the acquisition of two or three-dimensional spatial information have been proposed. However, these devices suffer a number of drawbacks Accordingly, the problem of developing more sophisticated microflow cytometers based on electrical impedance, optical detection and image analysis methods has received significant attention in the literature. This review describes some of the major advances made in the microfluidic cytometry field over the past ten years. The review focuses specifically on recent proposals for enhanced microfluidic focusing techniques and detection/analysis methods, respectively. Overall, the review provides a useful insight into the microflow cytometer technology field for both new and existing users.