The role of biogeography in shaping diversity of the intestinal microbiota in house mice
The role of biogeography in shaping diversity of the intestinal microbiota in house mice
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DOI：
10.1111/mec.12206
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发表时间：
2013-04-01
影响因子：
4.9
通讯作者：
Baines, John F.
中科院分区：
文献类型：
--
作者：
Linnenbrink, Miriam;Wang, Jun;Baines, John F.
栖息在哺乳动物肠道中的微生物群落在宿主生物学的各个方面发挥着重要作用。然而，鲜为人知的是，在这些社区的力量塑造的变化和他们对主机健身的影响。为了阐明宿主遗传学，传播和地理对个体之间微生物群落多样性的贡献，我们使用细菌16S rRNA基因的焦磷酸测序对来自西欧8个地点的121只家鼠的肠道微生物群落进行了调查。研究的寄主因子包括微卫星位点和线粒体DNA估计的种群结构、遗传距离和地理位置。为了确定宿主组织（粘膜）相关群落是否显示出与管腔不同的特性，检查了盲肠粘膜和内容物。我们确定拟杆菌属，罗宾逊和螺杆菌属的最丰富的属在盲肠内容物和粘膜相关的社区野生家鼠。总体而言，我们发现地理是解释肠道微生物群多样性模式的最重要因素，宿主种群结构和遗传距离的影响相对较弱。此外，宿主遗传距离的影响仅限于粘膜群落，这与该环境与宿主的耦合更密切一致。
The microbial communities inhabiting the mammalian intestinal tract play an important role in diverse aspects of host biology. However, little is known regarding the forces shaping variation in these communities and their influence on host fitness. To shed light on the contributions of host genetics, transmission and geography to diversity in microbial communities between individuals, we performed a survey of intestinal microbial communities in a panel of 121 house mice derived from eight locations across Western Europe using pyrosequencing of the bacterial 16S rRNA gene. The host factors studied included population structure estimated by microsatellite loci and mitochondrial DNA, genetic distance and geography. To determine whether host tissue (mucosa)-associated communities display properties distinct from those of the lumen, both the caecal mucosa and contents were examined. We identified Bacteroides, Robinsoniella and Helicobacter as the most abundant genera in both the caecal content and mucosa-associated communities of wild house mice. Overall, we found geography to be the most significant factor explaining patterns of diversity in the intestinal microbiota, with a comparatively weaker influence of host population structure and genetic distance. Furthermore, the influence of host genetic distance was limited to the mucosa communities, consistent with this environment being more intimately coupled to the host.