Mucosal priming with PEI/DNA complex and systemic boosting with recombinant TianTan vaccinia stimulate vigorous mucosal and systemic immune responses
Mucosal priming with PEI/DNA complex and systemic boosting with recombinant TianTan vaccinia stimulate vigorous mucosal and systemic immune responses
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DOI：
10.1016/j.vaccine.2006.12.020
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发表时间：
2007-03-30
期刊：
影响因子：
5.5
通讯作者：
Shao, Yiming
中科院分区：
文献类型：
--
作者：
Huang, Xianggang;Xu, Jianqing;Shao, Yiming
有效的HIV-1疫苗策略可能需要诱导和维持体液免疫和细胞免疫。我们在BALB/c小鼠中测试了一种新的引发-加强方法，即用PEI/DNA复合物中的10 μ g DNA质粒鼻内引发，并用107 PFU的表达HIV-1 Gag的复制型重组天坛痘苗病毒（rTTV）加强。用PEI/DNA复合物的鼻内引发在肺（p = 0.0445）和阴道（p = 0.0469）的粘膜部位引起比用裸DNA的鼻内引发显著更强的HIV特异性T细胞（p = 0.0358）和伊加免疫应答，尽管两者之后是相同的rTTV加强。此外，肌内加强与rTTV可以大大增强T细胞和抗体免疫反应所提出的鼻内引发。这些结果表明，用PEt/DNA复合物鼻内引发和用rTTV全身加强的组合是诱导T细胞和体液免疫应答的优选方案。
An effective vaccine strategy for HIV-1 will probably requires the induction and maintenance of both humoral and cellular immunity. We tested a new prime-boost approach of intranasal priming with 10 mu g DNA plasmid in the PEI/DNA complexes and boosting with 107 PFU of replicative recombinant TianTan vaccinia virus (rTTV) expressing HIV-1 Gag in BALB/c mice. Intranasal priming with PEI/DNA complexes elicited strikingly stronger HIV-specific T-cell (p = 0.0358) and IgA immune responses at mucosal sites of lung (p = 0.0445) and vaginal tract (p = 0.0469) than intranasal priming with naked DNA, though both are followed by the same rTTV boosting. Furthermore, an intramuscular boosting with rTTV could profoundly enhance both T-cell and antibody immune responses raised by intranasal priming. These results demonstrate that the combination of intrallasal priming with PEt/DNA complexes and systemic boosting with rTTV is a preferable regimen for induction of both T-cell and humoral immune responses.