THE CATECHOLAMINERGIC SYSTEM OF THE QUAIL BRAIN - IMMUNOCYTOCHEMICAL STUDIES OF DOPAMINE BETA-HYDROXYLASE AND TYROSINE-HYDROXYLASE
THE CATECHOLAMINERGIC SYSTEM OF THE QUAIL BRAIN - IMMUNOCYTOCHEMICAL STUDIES OF DOPAMINE BETA-HYDROXYLASE AND TYROSINE-HYDROXYLASE
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DOI：
10.1002/cne.903290206
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发表时间：
1993-03-08
影响因子：
2.5
通讯作者：
BALTHAZART, J
中科院分区：
文献类型：
--
作者：
BAILHACHE, T;BALTHAZART, J
通过免疫细胞化学研究了雄性和雌性日本鹌鹑大脑中儿茶酚胺生物合成中的两种关键酶多巴胺β-羟化酶和酪氨酸羟化酶的分布。显示多巴胺β-羟化酶和酪氨酸羟化酶免疫反应性的细胞或纤维被认为是去甲肾上腺素能或肾上腺素能的，而仅显示酪氨酸羟化酶免疫反应性的所有结构暂时被认为是多巴胺能的。在哺乳动物大脑中发现的主要多巴胺能和去甲肾上腺素能细胞群可以在日本鹌鹑中观察到，但结节漏斗多巴胺能细胞群除外。多巴胺β-羟化酶免疫反应性细胞仅在脑桥（蓝斑和蓝下核腹侧）和髓质（网状核区域）中发现。酪氨酸羟化酶免疫反应细胞分布更广泛，从视前区延伸至髓质水平。然而，它们大量存在于 Tsai 腹侧区域和被盖脚脑桥核、致密部，它们分别对应于哺乳动物的腹侧被盖区和黑质。在与生殖控制有关的几个对类固醇敏感的大脑区域中发现了高密度的多巴胺β-羟化酶和酪氨酸羟化酶免疫反应性纤维和点状结构。在视前区和伏隔核-终纹核区域，免疫阴性核周完全被免疫反应性纤维包围，形成篮状结构。鉴于其中一些细胞含有芳香酶，这些结构可能代表儿茶酚胺调节芳香酶活性的形态底物。与男性相比，多巴胺β-羟化酶免疫反应性纤维也存在于女性视前区的更大部分中。视前区去甲肾上腺素能神经支配的这种性别差异可能反映了该区域去甲肾上腺素含量的性别差异。
The distribution of dopamine beta-hydroxylase and tyrosine hydroxylase, two key enzymes in the biosynthesis of catecholamines, was investigated by immunocytochemistry in the brain of male and female Japanese quail. Cells or fibers showing dopamine beta-hydroxylase and tyrosine hydroxylase immunoreactivity were considered to be noradrenergic or adrenergic, while all structures showing only tyrosine hydroxylase immunoreactivity were tentatively considered to be dopaminergic. The major dopaminergic and noradrenergic cell groups that have been identified in the brain of mammals could be observed in the Japanese quail, with the exception of a tuberoinfundibular dopaminergic group. The dopamine beta-hydroxylase-immunoreactive cells were found exclusively in the pons (locus ceruleus and nucleus subceruleus ventralis) and in the medulla (area of the nucleus reticularis). The tyrosine hydroxylase-immunoreactive cells had a much wider distribution and extended from the preoptic area to the level of the medulla. They were, however, present in larger numbers in the area ventralis of Tsai and in the nucleus tegmenti pedunculo-pontinus, pars compacta, which respectively correspond to the ventral tegmental area and to the substantia nigra of mammals. A high density of dopamine beta-hydroxylase- and tyrosine hydroxylase-immunoreactive fibers and punctate structures was found in several steroid-sensitive brain regions that are implicated in the control of reproduction. In the preoptic area and in the region of the nucleus accumbens-nucleus stria terminalis, immunonegative perikarya were completely surrounded by immunoreactive fibers forming basket-like structures. Given that some of these cells contain the enzyme aromatase, these structures may represent the morphological substrate for a regulation of aromatase activity by catecholamines. The dopamine beta-hydroxylase-immunoreactive fibers were also present in a larger part of the preoptic area of females than in males. This sex difference in the noradrenergic innervation of the preoptic area presumably reflects the sex difference in norepinephrine content in this region.