Making artificial antibodies: A format for phage display of combinatorial heterodimeric arrays
Making artificial antibodies: A format for phage display of combinatorial heterodimeric arrays
复制标题
DOI：
10.1073/pnas.96.11.6025
复制
发表时间：
1999-05-25
影响因子：
11.1
通讯作者：
Janda, KD
中科院分区：
文献类型：
--
作者：
Gao, CS;Mao, SL;Janda, KD
基因MI蛋白(PVII)和基因IX蛋白(Pix)紧密结合在丝状噬菌体表面，与被广泛利用的PIII蛋白的末端相反。我们开发了一种噬菌体形式，其中抗体重链可变区和轻链可变区分别与pVII和pIX的氨基末端融合。值得注意的是，融合蛋白相互作用，在噬菌体表面形成了一个功能性的FV结合域。我们的方法将适用于可以形成组合异二聚体阵列的通用多肽和蛋白质文库的展示。因此，它代表着朝着人工抗体和选择新的生物活性迈出的第一步。
The gene MI protein (pVII) and gene IX protein (pIX) are associated closely on the surface of filamentous bacteriophage that is opposite of the end harboring the widely exploited pIII protein. We developed a phagemid format wherein antibody heavy- and light-chain variable regions were fused to the amino termini of pVII and pIX, respectively. Significantly, the fusion proteins interacted to form a functional Fv-binding domain on the phage surface. Our approach will be applicable to the display of generic peptide and protein libraries that can form combinatorial heterodimeric arrays. Consequently, it represents a first step toward artificial antibodies and the selection of novel biological activities.