Effect of serum and platelet-derived growth factor on chondrocytes grown in collagen gels.
Effect of serum and platelet-derived growth factor on chondrocytes grown in collagen gels.
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DOI：
10.1089/ten.1999.5.533
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发表时间：
1999-12
影响因子：
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通讯作者：
L. Weiser;M. Bhargava;E. Attia;P. Torzilli
中科院分区：
文献类型：
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作者：
L. Weiser;M. Bhargava;E. Attia;P. Torzilli
在这项体外研究中，细胞增殖、活力和形态；蛋白多糖（PG）合成；对胶原凝胶中培养的成熟牛软骨细胞在 15 天的时间内（第 3、6、9、12 和 15 天）评估凝胶收缩情况。通过改变凝胶和孵育介质中胎牛血清（1%和10%）和血小板衍生生长因子-BB（PDGF；0、10、50、100ng/ml）的浓度来改变凝胶内的环境。我们的结果表明，添加到凝胶中的血清或 PDGF 的量对细胞活力没有影响，在整个实验过程中 >95% 的细胞保持存活。随着时间的推移，所有组中的细胞数量均显着增加，在含有 10% 血清和较高浓度 PDGF 的凝胶中细胞增殖率较高。此外，在有或没有PDGF的情况下，血清的量显着影响凝胶收缩。含有 10% 血清的凝胶在第 10-12 天收缩，而含有 1% 血清的凝胶在实验过程中没有收缩。仅对于含有 1% 血清和 10 或 100 ng/ml PDGF 的凝胶，每个凝胶内的 PG 含量随孵育时间增加。然而，以每个细胞为基础，当仅使用血清时，PG含量随时间没有变化，并且添加PDGF后PG产生速率显着降低（9.1-27.8 pgPG/细胞/天）。细胞形态也受到PDGF的影响，细胞变得更加纺锤形。凝胶内的细胞排列似乎受凝胶收缩的影响最大。胶原凝胶可以作为组织工程目的的细胞载体。这些凝胶提供了软骨细胞可以增殖并产生基质的三维环境。我们已经展示了如何控制这种环境来影响凝胶收缩、细胞生长和 PG 产生的速率以及细胞形态，同时保持细胞活力。这些信息将有助于确定软骨细胞在胶原凝胶内生长并与细胞因子结合以创建理想的组织结构的条件。
In this in vitro study, cell proliferation, viability, and morphology; proteoglycan (PG) synthesis; and gel contraction were assessed over a 15-day period (on days 3, 6, 9, 12, and 15) for mature bovine chondrocytes cultured in collagen gels. The environment within the gel was varied by changing the concentration of fetal bovine serum (1% and 10%) and platelet-derived growth factor-BB (PDGF; 0, 10, 50, 100 ng/ml) within the gel and incubation media. Our results showed that the amount of serum or PDGF added to the gels had no effect on cell viability, with >95% of cells remaining alive throughout the experiment. There was a significant increase in cell number over time in all groups, with a higher rate of cell proliferation in gels containing 10% serum and higher concentrations of PDGF. In addition, the amount of serum significantly affected gel contraction with or without PDGF. Gels containing 10% serum contracted on day 10-12, while none of the gels containing 1% serum contracted over the course of the experiment. The PG content within each gel increased with incubation time only for the gels containing 1% serum, and 10 or 100 ng/ml of PDGF. However, on a per cell basis, there was no change in the PG content with time when only serum was used and a significant decrease in the rate of PG production with the addition of PDGF (9.1-27.8 pgPG/cell/day). Cell morphology was also affected by PDGF, with the cells becoming more spindle shaped. Cell alignment within the gels appeared to be most affected by gel contraction. Collagen gels can act as cell carriers for the purpose of tissue engineering. These gels provide a three-dimensional environment in which chondrocytes can proliferate and produce matrix. We have shown how this environment can be controlled to affect gel contraction, rates of cell growth and PG production, and cellular morphology while maintaining cell viability. This information will be useful in determining the conditions in which chondrocytes can be grown within collagen gels and combined with cytokines to create an ideal tissue construct.