ANTIBODY-RESPONSE FOLLOWING PROLONGED HYPOSENSITIZATION WITH DERMATOPHAGOIDES-PTERONYSSINUS EXTRACT
ANTIBODY-RESPONSE FOLLOWING PROLONGED HYPOSENSITIZATION WITH DERMATOPHAGOIDES-PTERONYSSINUS EXTRACT
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DOI：
10.1159/000232471
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
NUNN, AJ
中科院分区：
文献类型：
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作者：
CHAPMAN, MD;PLATTSMILLS, TAE;NUNN, AJ
采用抗原结合放射免疫测定法测定了对蝶蝶水提取物或安慰剂进行过低敏治疗的患者血清中针对蝶蝶的igg[免疫球蛋白]G、IgA和IgE抗体（ab）。从蝶翼龙培养物中获得过敏原F4P1，并用125i标记用于实验。用放射过敏吸收试验测定了鸡翼双歧杆菌的IgE抗体和鸡翼双歧杆菌的IgE抗体。放射免疫分析结果显示，翼虫治疗（DP）患者血清中IgG抗体选择性升高。29例DP患者中有27例治疗后血清中IgG抗体升高超过2倍，而29例安慰剂治疗患者中只有1例（P .mchlt）。0.001)。DP患者IgG抗体平均升高6倍，最高升高50倍。在治疗期间，DP患者或安慰剂患者的IgA抗体或IgE抗体水平变化不大。与安慰剂组相比，DP组患者的临床表现有显著改善（P < 0.01）。治疗后IgG抗体与治疗后症状评分无相关性，治疗过程中IgG抗体变化与症状评分变化无相关性。使用极低浓度的F4P1研究抗原结合试验的时间过程。抗原结合是缓慢的，即使存在过量的IgG抗体。IgG抗体在低敏化的作用进行了讨论。
An antigen-binding radioimmunoassay was used to measure Ig[immunoglobulin]G, IgA and IgE antibody (ab) to D. pteronyssinus in sera from patients who had been hyposensitized with aqueous D. pteronyssinus extract or placebo. An allergen, F4P1, was obtained from D. pteronyssinus culture and 125I-labeled for use in the assay. The radioallergosorbent test was also used to measure IgE ab to D. pteronyssinus and IgE ab to D. farinae. The radioimmunoassay results showed a selective rise in IgG ab in sera from D. pteronyssinus-treated (DP) patients. An increase of greater than 2-fold in IgG ab in post-treatment sera was found in 27 of 29 DP patients compared to 1 of 29 placebo-treated (placebo) patients (P .mchlt. 0.001). The mean rise in IgG ab in DP patients was 6-fold, and the maximum rise was 50-fold. There was little change in the levels of IgA ab or IgE ab in DP or placebo patients during treatment. The DP patients showed significant clinical improvement compared with the placebo patients (P < 0.01). No correlation was found between post-treatment IgG ab and post-treatment symptom scores, or between the changes in IgG ab and the changes in symptom scores during treatment. The time course of the antigen-binding assay was investigated using very low concentrations of F4P1. Antigen-binding was slow, even in the presence of an excess of IgG ab. The role of IgG ab in hyposensitization was discussed.