The effects of zygotic lethal mutations on female germ-line functions in Drosophila.
The effects of zygotic lethal mutations on female germ-line functions in Drosophila.
复制标题
合子致死突变对果蝇雌性种系功能的影响。
DOI：
10.1016/0012-1606(84)90297-5
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发表时间：
1984
影响因子：
2.7
通讯作者：
Mahowald,AP
中科院分区：
文献类型：
--
作者：
Perrimon,N;Engstrom,L;Mahowald,AP
许多突变时导致致命的基因位点也可能在卵子发生中发挥重要作用。使用显性雌性不育技术检查了 EMS 诱导的 48 个基因座合子致死突变对母体的影响。发现了三类效应。在第一组（48 人中的 13 人）中，没有检测到母体效应。第二组（48 个中的 20 个）表现出母体对卵子发生、胚胎发生或两者的影响。最后一组中的 13 只，在发育逐渐恶化之前只产生了一些卵。这表明野生型基因产物的持久性可以产生这种结果。第三组（48 个中的 15 个）在种系克隆中产生细胞致死性，这种效应可能与其在不可或缺的细胞功能中的作用有关。在种系克隆中发现了三个基因座，它们产生类似于母体效应突变的胚胎表型。讨论了这项研究对早期发育遗传分析的影响。
Many genetic loci that result in lethality when mutated may also have an essential role in oogenesis. The maternal effects of EMS-induced zygotic lethal mutations at 48 loci were examined using the dominant female-sterile technique. Three categories of effects were found. In the first group (13 out of 48), no maternal effect was detected. The second set (20 out of 48) exhibited maternal effects on oogenesis, embryogenesis, or both. In 13 of this last group, only a few eggs were produced before a progressive deterioration of development occurred. It is suggested that perdurance of the wild-type gene product could produce this result. The third group (15 out of 48) produced cell lethality in germ-line clones, an effect that may be related to their role in indispensable cell functions. Three loci were found which, in germ-line clones, produced embryonic phenotypes that resemble maternal effect mutations. The implications of this study for the genetic analysis of early development are discussed.