Immunolocalization of Nestin in the lizard Podarcis muralis indicates up-regulation during the process of tail regeneration and epidermal differentiation
Immunolocalization of Nestin in the lizard Podarcis muralis indicates up-regulation during the process of tail regeneration and epidermal differentiation
复制标题
DOI：
10.1016/j.aanat.2013.12.004
复制
发表时间：
2014-01-01
影响因子：
2.2
通讯作者：
Alibardi, Lorenzo
中科院分区：
文献类型：
--
作者：
Alibardi, Lorenzo
为了研究参与组织重塑和再生的一些蛋白质，利用免疫细胞化学方法在正常和再生的蜥蜴尾部中检测了干细胞标记物和动态细胞骨架蛋白巢蛋白。在正常尾巴的组织中，巢蛋白仅存在于鳞片的α层和结缔组织的稀疏细胞中。然而，在尾巴再生过程中，巢蛋白更常见于各种组织中，特别是在表皮和再生鳞片、肌肉、神经以及生长中的脊髓中。在再生胚基和衍生的结缔组织中可见零星的巢蛋白免疫阳性细胞。在表皮中，巢蛋白在位于形成鳞片的顶端α角质形成细胞的细胞增殖的主要位点表达，但在β细胞中消失。在再生肌肉中，巢蛋白存在于成肌细胞和伸长的肌管中，但在成熟肌肉中消失。巢蛋白出现在正在生长的神经和再生脊髓的一些神经元中。它存在于心尖室管膜细胞、周围神经和雪旺细胞中。动态细胞骨架蛋白巢蛋白似乎在细胞增殖和细胞骨架重塑的主要部位被激活，这是上皮再形成和新鳞片、肌肉和神经组织分化所需的。 (C) 2013 爱思唯尔有限公司。版权所有。
In order to study some of the proteins involved in tissue remodeling and regeneration, the stem cell marker and dynamic cytoskeletal protein nestin have been detected using immunocytochemistry in normal and regenerating tail of lizard. In tissues of the normal tail, nestin is only present in the alphalayer of scales and in sparse cells of connective tissues. However, during tail regeneration, nestin is more commonly detected in various tissues, especially in the epidermis and regenerating scales, muscles, nerves and in the growing spinal cord. Sporadic nestin immunopositive cells are seen in the regenerating blastema and derived connective tissues. In the epidermis, nestin is expressed in the main sites of cell proliferation located in the apical alpha-keratinocytes of the forming scales, but disappears in beta-cells. In the regenerating muscles, nestin is seen in myoblasts and elongating myotubes, but disappears in mature muscles. Nestin appears in the growing nerves and in a few neurons of the regenerating spinal cord. It is present in the apical ependymal cells and in peripheral nerves and Schwann cells. The dynamic cytoskeletal protein nestin appears to be activated in the main sites of cell proliferation and cytoskeletal remodeling needed for re-epithelization and the differentiation of new scales, muscles and nervous tissue. (C) 2013 Elsevier GmbH. All rights reserved.