Imaging phospholipid conformational disorder and packing in giant multilamellar liposome by confocal Raman microspectroscopy
Imaging phospholipid conformational disorder and packing in giant multilamellar liposome by confocal Raman microspectroscopy
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DOI：
10.1016/j.saa.2017.06.060
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发表时间：
2017-12-05
影响因子：
4.4
通讯作者：
Yamamoto, Tatsuyuki
中科院分区：
文献类型：
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作者：
Noothalapati, Hemanth;Iwasaki, Keita;Yamamoto, Tatsuyuki
脂质体是封闭的磷脂双层系统，在基础细胞生物学、药学和医学中具有深远的应用。根据组成（纯磷脂或磷脂混合物，胆固醇的存在）和制备方案;链内和链间分子相互作用不同，导致脂质体质量（顺序和包装）发生变化。到目前为止，不可能以直接的方式对脂质体内的构象紊乱和堆积密度进行成像。在这项研究中，我们利用共焦拉曼显微光谱可视化的结构紊乱和packingefficiency内的一个巨大的多层脂质体模型，主要集中在三个区域的振动光谱（C-C伸缩，C-H变形和C-H伸缩）。我们估计的性质，如反/左异构体和横向包装概率。有趣的是，我们的拉曼成像研究揭示了凝胶相丰富的领域和巨大的多层脂质体内的异质性横向包装。(C)2017爱思唯尔B. V.保留所有权利。
Liposomes are closed phospholipid bilayer systems that have profound applications in fundamental cell biology, pharmaceutics and medicine. Depending on the composition (pure or mixture of phospholipids, presence of cholesterol) and preparation protocol; intra- and inter-chain molecular interactions vary leading to changes in the quality (order and packing) of liposomes. So far it is not possible to image conformational disorders and packing densities within a liposome in a straightforward manner. In this study, we utilized confocal Raman microspectroscopy to visualize structural disorders and packingefficiency within a giant multilamellar liposome model by focusing mainly on three regions in the vibrational spectrum (C-C stretching, C-H deformation and C-H stretching). We estimated properties such as trans/gauche isomers and lateral packing probability. Interestingly, our Raman imaging studies revealed gel phase rich domains and heterogeneous lateral packing within the giant multilamellar liposome. (C) 2017 Elsevier B.V. All rights reserved.