PRIMARY STRUCTURE AND FUNCTIONAL EXPRESSION OF A CYCLIC NUCLEOTIDE-GATED CHANNEL FROM RABBIT AORTA
PRIMARY STRUCTURE AND FUNCTIONAL EXPRESSION OF A CYCLIC NUCLEOTIDE-GATED CHANNEL FROM RABBIT AORTA
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DOI：
10.1016/0014-5793(93)80209-d
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发表时间：
1993-08-23
期刊：
影响因子：
3.5
通讯作者：
HOFMANN, F
中科院分区：
文献类型：
--
作者：
BIEL, M;ALTENHOFEN, W;HOFMANN, F
用聚合酶链式反应从心脏、主动脉、窦房结、小脑、C细胞和肾脏的c DNA中扩增出环核苷酸门控通道(CNG通道)的特异性序列。通过对兔主动脉CNG通道基因的克隆和序列分析，推导出该通道的完整氨基酸序列。由该基因合成的RNA可诱导非洲爪哇卵母细胞形成功能性CNG通道。
Sequences specific for cyclic nucleotide-gated channels (CNG channels) have been amplified by PCR from cDNA of heart, aorta, sino-atrial node, cerebellum, C-cells and kidney. The complete amino acid sequence of a CNG channel from rabbit aorta has been deduced by cloning and sequence analysis of the cDNA. Synthetic RNA derived from this cDNA induces the formation of a functional CNG channel in Xenopus oocytes.