Red blood cell deformation in microconfined flow
Red blood cell deformation in microconfined flow
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DOI：
10.1039/b904584h
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发表时间：
2009-09
期刊：
影响因子：
3.4
通讯作者：
G. Tomaiuolo;M. Simeone;V. Martinelli;B. Rotoli;S. Guido
中科院分区：
文献类型：
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作者：
G. Tomaiuolo;M. Simeone;V. Martinelli;B. Rotoli;S. Guido
在这项工作中，我们报告了红细胞（RBC）悬浮液在直径与体外细胞大小相当的微血管中流动的系统流体动力学研究。通过使用高速视频显微镜和图像分析，我们首次同时测定了在大范围压降下与红细胞膜变形性相关的细胞速度和形状参数，并首次与文献中的理论结果进行了定量比较。结果与预测的轴对称形状吻合良好，随着细胞速度的增加，轴对称形状趋向于明显的子弹样渐近结构。这项工作的一个潜在应用是设计基于流动的设备来研究影响细胞变形性的病理条件，从而使我们能够克服经典静态方法的局限性，例如微管抽吸，不适合处理大量细胞。
In this work, we report on a systematic fluidodynamic investigation of red blood cell (RBC) suspensions flowing in microcapillaries with diameters comparable to the cell size in vitro. By using high-speed video microscopy and image analysis, we provide the first simultaneous determination of both cell velocity and shape parameters related to RBC membrane deformability over an extended range of pressure drops, and the first quantitative comparison with theoretical results from the literature. Good agreement was found with the predicted axisymmetric shapes tending towards an apparent bullet-like asymptotic configuration at increasing cell velocity. A potential application of this work is in the design of flow-based devices to study the pathological conditions affecting cell deformability, thus allowing us to overcome the limits of classical static methods, such as micropipette aspiration, which are not suitable for handling a large number of cells.