Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats
Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats
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DOI：
10.1186/s12985-014-0196-x
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发表时间：
2014-11-25
期刊：
影响因子：
4.8
通讯作者：
Hoffmann, Bernd
中科院分区：
文献类型：
--
作者：
Fischer, Melina;Freuling, Conrad M.;Hoffmann, Bernd
背景资料：在蝙蝠狂犬病病毒主动监测的框架下，采用新开发的通用型泛狂犬病病毒实时逆转录酶PCR（RT-qPCR）对德国（N = 2297）和丹麦（N = 134）食虫蝙蝠的口咽拭子进行了研究。值得注意的是，阳性样品的测序没有确认蝙蝠相关的狂犬病毒的存在，但揭示了9个不同的新的弹状病毒相关的sequence.Conclusions：几个新的弹状病毒相关的序列在德国和丹麦的食虫蝙蝠检测。结果还证明，新的通用泛狂犬病毒RT-qPCR提供了非常广泛的检测范围，允许收集关于弹状病毒科内广泛和复杂多样性的进一步有价值的数据。
Background: In the frame of active lyssavirus surveillance in bats, oropharyngeal swabs from German (N = 2297) and Danish (N = 134) insectivorous bats were investigated using a newly developed generic pan-lyssavirus real-time reverse transcriptase PCR (RT-qPCR).Findings: In total, 15 RT-qPCR positive swabs were detected. Remarkably, sequencing of positive samples did not confirm the presence of bat associated lyssaviruses but revealed nine distinct novel rhabdovirus-related sequences.Conclusions: Several novel rhabdovirus-related sequences were detected both in German and Danish insectivorous bats. The results also prove that the novel generic pan-lyssavirus RT-qPCR offers a very broad detection range that allows the collection of further valuable data concerning the broad and complex diversity within the family Rhabdoviridae.