Production of trans-10, cis-12 conjugated linoleic acid in rice
Production of trans-10, cis-12 conjugated linoleic acid in rice
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DOI：
10.1007/s11248-005-3736-8
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发表时间：
2006-02-01
影响因子：
3
通讯作者：
Imamura, J
中科院分区：
文献类型：
--
作者：
Kohno-Murase, J;Iwabuchi, M;Imamura, J
共轭亚油酸（CLA）具有抗肿瘤、抗动脉粥样硬化、调节免疫系统和脂质代谢等作用。为了产生可以积累CLA的转基因水稻植物，引入可以将亚油酸转化为反式-10，顺式-12 CLA的亚油酸异构酶基因，并在来自油质蛋白和球蛋白基因的种子特异性启动子的控制下表达。用气相色谱法分析了转基因水稻籽粒的脂肪酸组成。虽然有没有明显的差异，从转化与未转化植物的种子之间的脂肪酸组成，反式-10，顺式-12共轭亚油酸甲酯，这是不存在于未转化植物的种子中，被发现在转化植物的峰。在携带油质蛋白启动子的种子中，反式-10，顺式-12 CLA平均占总脂肪酸的1.3%（w/w），而在携带球蛋白启动子的种子中为0.01%（w/w）。此外，大约70%和28%的CLA异构体的总量分别存在于三酰甘油和游离脂肪酸馏分中。这些结果证明了在作物中产生具有新生理活性的植物油脂肪酸组分的能力。
Conjugated linoleic acid (CLA) has anti-carcinogenic and anti-atherosclerosis activity, and modulatory effects on the immune system and lipid metabolism. To produce a transgenic rice plant that can accumulate CLA, a linoleate isomerase gene that can convert linoleic acid to trans-10, cis-12 CLA was introduced and expressed under the control of seed-specific promoters from the oleosin and globulin genes. The fatty acid composition of the transgenic rice grain was analyzed by gas chromatography. Although there was no clear difference in the fatty acid composition between seeds from transformed versus untransformed plants, a peak of trans-10, cis-12 CLA methyl ester, which was not present in seeds from untransformed plants, was found in transformed plants. The trans-10, cis-12 CLA comprised an average of 1.3% (w/w) of the total fatty acids in seeds carrying the oleosin promoter in comparison to 0.01% (w/w) in seeds carrying the globulin promoter. In addition, approximately 70 and 28% of the total amount of the CLA isomer were present in the triacylglycerol and free fatty acid fractions, respectively. These results demonstrate the ability to produce fatty acid components of vegetable oils with novel physiological activities in crops.