Intravenous basic fibroblast growth factor decreases brain injury resulting from focal ischemia in cats
Intravenous basic fibroblast growth factor decreases brain injury resulting from focal ischemia in cats
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DOI：
10.1161/01.str.28.3.609
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发表时间：
1997-03-01
期刊：
影响因子：
8.3
通讯作者：
Traystman, RJ
中科院分区：
文献类型：
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作者：
Bethel, A;Kirsch, JR;Traystman, RJ
背景和目的我们验证了在永久性局灶性脑缺血4小时内静脉注射碱性成纤维细胞生长因子（bFGF）对急性脑损伤的影响。对照猫接受稀释剂（n=14）。实验猫在缺血开始后60分钟开始静脉注射bFGF，剂量分别为5（n = 13），50（n=13），或250 μ g/kg/h（n=9），并持续至实验结束。给药前，所有组。同样，在MCA闭塞期间，bFGF给药导致同侧尾状核或下颞叶皮质的血流量没有差异。三苯基四氮唑测定的同侧大脑皮层损伤体积（对照组，40+/-7%; bFGF 5 μ g/kg/h，22+/-5%; bFGF 50 μ g/kg/h，26+/-7%; bFGF 255 μ g/kg/h，同侧大脑皮层23+/-6%;平均值+/-SEM）在bFGF治疗的猫中较小。各组尾状核损伤体积无差异（对照组，29+/-8%; bFGF 5 μ g/kg/h，29+/-8%; bFGF 50 μ g/kg/h，21+/-7%; bFGF 250 μ g/kg/h，32+/-7%同侧尾状核）。体感诱发电位振幅降低相似（至
Background and Purpose We tested the hypothesis that intravenous administration of basic fibroblast growth factor (bFGF) during 4 hours of permanent focal ischemia would affect acute brain injury.Methods Halothane-anesthetized cats underwent left middle cerebral artery (MCA) occlusion for 4 hours. Control cats received diluent (n=14). Experimental cats were treated with bFGF ata rate of 5 (n=13), 50 (n=13), or 250 mu g/kg per hour (n=9) intravenously beginning 60 minutes after initiation of ischemia and continuing until the end of the protocol.Results As measured by the microsphere method, blood flow to ipsilateral caudate nucleus and ipsilateral inferior temporal cortex was decreased similarly during ischemia, before drug administration, in all groups. Likewise, there was no difference in blood flow to ipsilateral caudate nucleus or inferior temporal cortex as a result of bFGF administration during MCA occlusion. Triphenyltetrazolium-determined injury volume of the ipsilateral cerebral cortex (control, 40+/-7%; bFGF 5 mu g/kg per hour, 22+/-5%; bFGF 50 mu g/kg per hour, 26+/-7%; bFGF 255 mu g/kg per hour, 23+/-6% of ipsilateral cerebral cortex; mean+/-SEM) was less in cats treated with bFGF. There was no difference among groups in injury volume to caudate nucleus (control, 29+/-8%; bFGF 5 mu g/kg per hour, 29+/-8%; bFGF 50 mu g/kg per hour, 21+/-7%; bFGF 250 mu g/kg per hour, 32+/-7% of ipsilateral caudate nucleus). Somatosensory evoked potential amplitude decreased similarly (to