Millimeter wave radiation induces apoptosis via affecting the ratio of Bax/Bcl-2 in SW1353 human chondrosarcoma cells
Millimeter wave radiation induces apoptosis via affecting the ratio of Bax/Bcl-2 in SW1353 human chondrosarcoma cells
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DOI：
10.3892/or.2011.1558
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发表时间：
2012-03-01
期刊：
影响因子：
4.2
通讯作者：
Liu, Xianxiang
中科院分区：
文献类型：
--
作者：
Li, Xihai;Yei, Hongzhi;Liu, Xianxiang
毫米波辐射对各种类型的恶性肿瘤的有效性和安全性已被证明。然而，毫米波辐射对细胞凋亡的潜在影响机制仍不清楚。本研究旨在研究毫米波辐射对SW1353细胞凋亡和线粒体膜电位的影响，并通过检测细胞中Bcl2家族蛋白(Bcl2、Bax)、caspase-9和caspase-3的表达，探讨毫米波辐射诱导细胞凋亡的分子机制。我们发现毫米波辐射抑制了SW1353细胞的活力，表明毫米波辐射诱导细胞凋亡，并以时间依赖的方式降低细胞活力。此外，我们还观察到，毫米波辐射处理细胞后，线粒体膜电位显著降低，促凋亡基因Bax、caspase-9和caspase-3表达上调，但抗凋亡基因Bcl2的表达水平没有明显变化。提示毫米波辐射可能通过影响Bax/Bcl2的比值而诱导SW1353细胞发生凋亡。
The efficacy and safety of millimeter wave radiation has been proven for various types of malignant tumors. However, the mechanisms underlying effects of millimeter wave radiation on apoptosis are still unclear. The present study was undertaken to examine the effects of millimeter wave radiation on cell apoptosis and mitochondrial membrane potential, and to determine the molecular mechanism of millimeter wave radiation-induced apoptosis by investigating the expression of Bcl-2 family proteins (Bcl-2, Bax), caspase-9 and caspase-3 in SW1353 cells. We found that millimeter wave radiation suppressed the viability of SW 1353 cells, demonstrating that millimeter wave radiation induced cell apoptosis and reduced cell viability in a time-dependent manner. Furthermore, we observed that treatment of cells with millimeter wave radiation significantly induced loss of mitochondrial membrane potential, upregulated proapoptotic Bax, caspase-9 and caspase-3, but did not significantly change levels of antiapoptotic Bcl-2. These data suggested that millimeter wave radiation may induce apoptosis via affecting the ratio of Bax/Bcl-2 in SW1353 cells.