Characterisation of a barley (Hordeum vulgare L.) homologue of the Arabidopsis flowering time regulator GIGANTEA
Characterisation of a barley (Hordeum vulgare L.) homologue of the Arabidopsis flowering time regulator GIGANTEA
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DOI：
10.1007/s00122-004-1912-5
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发表时间：
2005-03-01
影响因子：
5.4
通讯作者：
Laurie, DA
中科院分区：
文献类型：
--
作者：
Dunford, RP;Griffiths, S;Laurie, DA
分离与拟南芥开花时间调节剂GIGANTEA同源的大麦cDNA和基因组克隆。遗传作图表明，GIGANTEA在大麦（3 HS）和水稻（1 S）中以单拷贝基因存在，而在玉米（3S和8 S）中存在两个拷贝，其位置与先前的比较作图研究一致。大麦肽与水稻和拟南芥的相似性分别为94%和79%。大麦基因（HvGI）的北方和半定量RT-PCR分析表明，存在一个单一的mRNA种类，在短日照（8 h光照）下黎明后6 h和9 h之间出现表达高峰，在长日照（16 h光照）下黎明后15 h出现表达高峰。这种行为与拟南芥和水稻中所见的相似，表明序列和表达模式非常保守。与影响开花时间（抽穗期）的QTL的地图位置的对应关系的缺乏表明，在HvGI的变化不提供光周期响应的适应性变化的主要来源。
Barley cDNA and genomic clones homologous to the Arabidopsis flowering time regulator GIGANTEA were isolated. Genetic mapping showed that GIGANTEA is present as a single copy gene in barley (3HS) and rice (1S), while two copies are present in maize (3S and 8S) at locations consistent with previous comparative mapping studies. Comparison of the barley peptide with rice and Arabidopsis gave 94% and 79% similarity, respectively. Northern and semi-quantitative RT-PCR analysis of the barley gene (HvGI) showed the presence of a single mRNA species, with a peak of expression between 6 h and 9 h after dawn in short days (8 h light) and a peak 15 h after dawn in long days (16 h light). This behaviour is similar to that seen in Arabidopsis and rice, showing that sequence and expression pattern were well conserved. A lack of correspondence with the map positions of QTL affecting flowering time (heading date) suggests that variation at HvGI does not provide a major source of adaptive variation in photoperiod response.