MOSQUITO SALIVARY-GLAND ANTIGENS IDENTIFIED BY CIRCULATING HUMAN-ANTIBODIES
MOSQUITO SALIVARY-GLAND ANTIGENS IDENTIFIED BY CIRCULATING HUMAN-ANTIBODIES
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DOI：
10.1001/archderm.125.2.219
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发表时间：
1989-02-01
影响因子：
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通讯作者：
LEONARDI, C
中科院分区：
文献类型：
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作者：
PENNEYS, NS;NAYAR, JK;LEONARDI, C
从实验室饲养的佛罗里达南部常见物种品系的雌性蚊子中取出唾液腺。通过变性电泳从这些腺体的匀浆中分离蛋白质抗原，并将其转移到硝酸纤维素中，用于与从人类志愿者获得的血清样品进行免疫印迹。通过人血清中的抗体鉴定了每个种属的分子量范围广泛（14至126千道尔顿）的抗原谱。种属独特和种属共有的抗原都存在。这些研究的结果表明，蚊子叮咬的体液反应是复杂的，有许多抗原引起抗体反应。由于每个人都有自己独特的蚊子暴露史，因此对特定免疫原的脱敏似乎不太可能在多次暴露于各种蚊子物种的临床情况下提供保护。
Salivary glands were removed from female mosquitoes of laboratory-reared strains of species common to southern Florida. Protein antigens were isolated from homogenates of these glands by denaturing electrophoresis and transferred to nitrocellulose for immunoblotting with serum samples obtained from human volunteers. A spectrum of antigens with a wide range of molecular weights (14 to 126 kilodaltons) were identified by antibodies in human serum for each species. Both species-unique and species-shared antigens were present. The results of these studies indicate that the humoral response to mosquito bite is complex, with a multitude of antigens provoking antibody responses. Since each individual has his or her own unique exposure history to mosquitoes, it seems unlikely that densensitization to a specific immunogen will confer protection in clinical situations in which multiple exposures to a variety of mosquito species occur.