Genotypic diversity of Haemophilus parasuis field strains
Genotypic diversity of Haemophilus parasuis field strains
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DOI：
10.1128/aem.02834-05
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发表时间：
2006-06-01
影响因子：
4.4
通讯作者：
Aragon, V.
中科院分区：
文献类型：
--
作者：
Olvera, A.;Calsamiglia, M.;Aragon, V.
副猪嗜血杆菌（Haernophilus parasuis）是猪的格拉瑟病（Glasser's disease）和其他临床病症的原因。它也可以从健康猪的上呼吸道中分离出来，分离株的毒力可能存在显著差异。在这项工作中，从60 kDa的热休克蛋白（Hsp60）基因的部分序列进行了评估作为流行病学标志物。对103株H.副猪链球菌和其他相关物种，以获得更好的菌株分类，并检查与毒力的相关性。并与肠杆菌重复基因间共有序列PCR的结果进行比较。结果表明，hsp60是一个可靠的流行病学研究指标。它的序列分析是一种比指纹识别方法更好的方法。此外，hsp60和16SrRNA基因序列的分析显示，存在一个单独的谱系的强毒株，并表明发生横向基因转移之间的H。副猪链球菌和放线杆菌菌株。
Haernophilus parasuis is the cause of Glasser's disease and other clinical disorders in pigs. It can also be isolated from the upper respiratory tracts of healthy pigs, and isolates can have significant differences in virulence. In this work, a partial sequence from the 60-kDa heat shock protein (Hsp60) gene was assessed as an epidemiological marker. We analyzed partial sequences of hsp60 and 16S rRNA genes from 103 strains of H. parasuis and other related species to obtain a better classification of the strains and examine the correlation with virulence. The results were compared with those obtained by enterobacterial repetitive intergenic consensus PCR. Our results showed that hsp60 is a reliable marker for epidemiological studies of H. parasuis and that the analysis of its sequence is a better approach than fingerprinting methods. Furthermore, the analysis of the hsp60 and 16S rRNA gene sequences revealed the presence of a separate lineage of virulent strains and indicated the occurrence of lateral gene transfer among H. parasuis and Actinobacillus strains.