Atomic Force Microscopy: A Powerful Tool to Address Scaffold Design in Tissue Engineering.
Atomic Force Microscopy: A Powerful Tool to Address Scaffold Design in Tissue Engineering.
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DOI：
10.3390/jfb8010007
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发表时间：
2017-02-13
影响因子：
4.8
通讯作者：
Ambrosio L
中科院分区：
文献类型：
--
作者：
Marrese M;Guarino V;Ambrosio L
目前，功能聚合物是广泛的生物和生物医学应用的基本组成部分，包括分子释放、组织工程、生物传感和医学成像。这些领域的进步是由一系列具有受控物理和生物相互作用性质的生物可降解聚合物的使用推动的。在这种背景下，原子力显微镜(AFM)等显微技术正在成为深入研究微观和亚微米尺度的形态和结构特性，以评估生物材料的物理化学性质与生物响应之间的及时关系的基本工具。特别是，原子力显微镜不仅是一种筛选表面形貌的工具，而且可以为了解表面和界面性质做出重大贡献，从而有助于在微米和纳米尺度上优化生物材料的性能、工艺、物理和化学性质。这是可能的，通过利用纳米技术的最新发现应用于软物质，如原子力光谱，通过力曲线测量表面力。通过针尖-样品的局部相互作用，可以收集一些信息，如弹性、粘弹性、表面电荷密度和润湿性。本文综述了原子力显微镜技术和成像技术的最新进展，从不同形式的聚合物器件(即纤维、薄膜或颗粒)的表征出发，评述了操作模式、先进工具的实施及其在生物材料科学中的应用现状。
Functional polymers currently represent a basic component of a large range of biological and biomedical applications including molecular release, tissue engineering, bio-sensing and medical imaging. Advancements in these fields are driven by the use of a wide set of biodegradable polymers with controlled physical and bio-interactive properties. In this context, microscopy techniques such as Atomic Force Microscopy (AFM) are emerging as fundamental tools to deeply investigate morphology and structural properties at micro and sub-micrometric scale, in order to evaluate the in time relationship between physicochemical properties of biomaterials and biological response. In particular, AFM is not only a mere tool for screening surface topography, but may offer a significant contribution to understand surface and interface properties, thus concurring to the optimization of biomaterials performance, processes, physical and chemical properties at the micro and nanoscale. This is possible by capitalizing the recent discoveries in nanotechnologies applied to soft matter such as atomic force spectroscopy to measure surface forces through force curves. By tip-sample local interactions, several information can be collected such as elasticity, viscoelasticity, surface charge densities and wettability. This paper overviews recent developments in AFM technology and imaging techniques by remarking differences in operational modes, the implementation of advanced tools and their current application in biomaterials science, in terms of characterization of polymeric devices in different forms (i.e., fibres, films or particles).