Nuclear localization of a new c-cbl related protein, CARP 90, during in vivo thymic apoptosis in mice
Nuclear localization of a new c-cbl related protein, CARP 90, during in vivo thymic apoptosis in mice
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DOI：
10.1038/sj.cdd.4400542
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发表时间：
1999-07-01
影响因子：
12.4
通讯作者：
Régnier, D
中科院分区：
文献类型：
--
作者：
Denis, G;Mandard, S;Régnier, D
本研究探讨了c-cbl原癌基因在氢化可的松治疗后体内胸腺细胞凋亡中的作用。在未经处理的小鼠胸腺中，少量髓质和皮质胸腺细胞表达p120(cbl)，主要在细胞质中，在皮质中，它们的数量和分布与凋亡细胞相似，TUNEL染色证实，当氢化可的松触发细胞凋亡时，cbl的表达迅速增加，这种cbl特异性免疫染色在细胞核中检测到，这是由于cbl相关的90 kDa蛋白（CARP 90）。这些结果表明，c-cbl产物可能定位于细胞核，并可能作为胸腺细胞凋亡的调节因子参与其中。
This study investigates the involvement of the c-cbl protooncogene in thymocyte apoptosis occuring in vivo after hydrocortisone treatment. In the thymus of untreated mice, a few medullary and cortical thymocytes expressed p120(cbl), mainly in the cytoplasm, In the cortex, their number and distribution resemble that of apoptotic cells evidenced by TUNEL staining, The expression of Cbl is rapidly increased when apoptosis is triggered by hydrocortisone, This Cbl-specific immunostaining was detected in the nucleus and is due to a Cbl-related 90 kDa protein (CARP 90), These results show that a c-cbl product could localize in the nucleus and suggest that it could be involved as a regulator of thymic apoptosis.