Membrane fluidity is involved in the regulation of heat stress induced secondary metabolism in Ganoderma lucidum
Membrane fluidity is involved in the regulation of heat stress induced secondary metabolism in Ganoderma lucidum
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膜流动性参与热应激诱导的灵芝次生代谢调节
DOI：
10.1111/1462-2920.13693
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发表时间：
2017-04-01
影响因子：
5.1
通讯作者：
Zhao, Ming-Wen
中科院分区：
文献类型：
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作者：
Liu, Yong-Nan;Zhang, Tian-Jun;Zhao, Ming-Wen
灵芝已成为评价环境因子如何调控担子菌次生代谢的潜在模式系统。热应激是重要的环境因子之一。以前有报道，HS可以诱导灵芝酸（GA）的生物合成。在这项研究中，我们发现，HS增加GA的生物合成，也显着增加细胞膜流动性。此外，我们的研究结果表明，加入膜硬化剂二甲基亚砜（DMSO）可以恢复增加GA的生物合成引起的HS。这些结果表明，膜流动性的增加与HS诱导的GA生物合成。进一步的证据表明，GA含量在D9去沉默菌株中降低，并且可以通过添加膜流化剂苯甲醇（BA）恢复到WT水平。相比之下，GA含量在D9des过表达菌株中增加，并且可以通过添加DMSO恢复到WT水平。此外，在WT和D9去沉默菌株中，HS诱导的膜流动性和GA生物合成都可以被DMSO逆转。据我们所知，这是第一个报告表明，膜流动性参与调节热胁迫诱导的丝状真菌次生代谢。
Ganoderma lucidum has become a potential model system for evaluating how environmental factors regulate the secondary metabolism of basidiomycetes. Heat stress (HS) is one of the most important environmental factors. It was previously reported that HS could induce the biosynthesis of ganoderic acids (GA). In this study, we found that HS increased GA biosynthesis and also significantly increased cell membrane fluidity. Furthermore, our results showed that addition of the membrane rigidifier dimethylsulfoxide (DMSO) could revert the increased GA biosynthesis elicited by HS. These results indicate that an increase in membrane fluidity is associated with HS-induced GA biosynthesis. Further evidence showed that the GA content was decreased in D9des-silenced strains and could be reverted to WT levels by addition of the membrane fluidizer benzyl alcohol (BA). In contrast, GA content was increased in D9des-overexpression strains and could be reverted to WT levels by the addition of DMSO. Furthermore, both membrane fluidity and GA biosynthesis induced by HS could be reverted by DMSO in WT and D9des-silenced strains. To the best of our knowledge, this is the first report demonstrating that membrane fluidity is involved in the regulation of heat stress induced secondary metabolism in filamentous fungi.