Upstream regulatory regions required to stabilize binding to the TATA sequence in an adenovirus early promoter.
Upstream regulatory regions required to stabilize binding to the TATA sequence in an adenovirus early promoter.
复制标题
上游调控区需要稳定与腺病毒早期启动子中 TATA 序列的结合。
DOI：
10.1093/nar/15.20.8367
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发表时间：
1987
影响因子：
14.9
通讯作者：
Gaynor,R
中科院分区：
文献类型：
--
作者：
Garcia,J;Wu,F;Gaynor,R
在5个早期腺病毒启动子中，早期3区（E3）启动子是E1A蛋白诱导最强烈的启动子之一。为了鉴定参与basa1和ela诱导的E3启动子转录调控的细胞蛋白，使用部分纯化的Hela细胞提取物进行ONase I足迹分析。E3启动子的四个区域作为细胞蛋白质的结合域。这些区域分布在- 156至- 179（位点IV）， - 83至- 103（位点III）， - 47至- 67（位点II）和- 16至- 37（位点I）之间，相对于转录开始。对每个结合域DNA序列的检查表明，III位点可能是激活蛋白1 （AP-1）的结合位点，II位点是环AMP调节元件结合蛋白（CREB）的结合位点，I位点是TATA结合因子的结合位点。结合位点II或III的因子足以稳定与TATA序列（位点I）的结合。诱变研究表明，除了位点I外，位点II和III也是基础转录和ela诱导转录所必需的。这些结果表明，多个细胞因子参与了E3启动子的基础和eia诱导的转录调控，并且两个上游区域中的任何一个都能够稳定因子与TATA序列的结合。
Of the five early adenovirus promoters, the early region 3 (E3) promoter is one of the most strongly induced by the E1A protein. To identify cellular proteins involved in both the basa1 and ElA-induced transcriptional regulation of the E3 promoter, ONase I footprinting using partially purified Hela cell extracts was performed. Four regions of the E3 promoter serve as binding domains for cellular proteins. These regions are found between −156 to −179 (site IV), −83 to −103 (site III), −47 to −67 (site II), and −16 to −37 (site I), relative to the start of transcription. Examination of the DNA sequences in each binding domain suggests that site III likely serves as a binding site for activator protein 1 (AP-1), site II for the cyclic AMP regulatory element binding protein (CREB), and site I for a TATA binding factor. The factors binding to either site II or III were sufficient to stabilize binding to the TATA sequence (site I). Mutagenesis studies Indicated that both sites II and III, in addition to site I, are needed for complete basal and ElA-induced transcription. These results suggest that multiple cellular factors are Involved in both the basal and EIA-induced transcriptional regulation of the E3 promoter, and that either of two upstream regions are capable of stabilizing factor binding to the TATA sequence.