Cloning, expression, and ontogeny of mouse organic anion-transporting polypeptide-5, a kidney-specific organic anion transporter.
Cloning, expression, and ontogeny of mouse organic anion-transporting polypeptide-5, a kidney-specific organic anion transporter.
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小鼠有机阴离子转运多肽-5（一种肾脏特异性有机阴离子转运蛋白）的克隆、表达和个体发育。
DOI：
10.1006/bbrc.2000.4072
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Klaassen,CD
中科院分区：
文献类型：
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作者：
Choudhuri,S;Ogura,K;Klaassen,CD
小鼠有机阴离子转运多肽（命名为小鼠Oatp-5）的全长编码序列已从小鼠肾脏cDNA文库中克隆。通过 Capsite 克隆对 Oatp-5 cDNA 的 5'-非翻译区 (5'-UTR) 进行分析，揭示了两个可能相距 4 bp 的转录起始位点。 Oatp-5 cDNA 的 3'-非翻译区 (3'-UTR) 包含早期聚腺苷酸化信号，表明具有不同 3'-UTR 长度的 mRNA 可能共存。推导的小鼠Oatp-5蛋白的氨基酸序列包含670个氨基酸，并具有10个假定的跨膜结构域、多个潜在的糖基化和磷酸化位点。组织特异性表达研究表明小鼠 Oatp-5 仅在肾脏中表达。发育表达研究表明，小鼠肾脏中Oatp-5 mRNA在出生后至少3周内无显着表达，而Oatp-5 mRNA表达在出生后6周以上才达到成人水平。系统发育分析表明小鼠 Oatp-5 是大鼠 Oatp-5 的直系同源物。
The full-length coding sequence of mouse organic anion-transporting polypeptide (designated mouse Oatp-5) has been cloned from mouse kidney cDNA library. Analysis of the 5′-untranslated region (5′-UTR) of Oatp-5 cDNA through capsite cloning reveals two possible transcription start sites that are 4-bp apart. The 3′-untranslated region (3′-UTR) of Oatp-5 cDNA contains an early polyadenylation signal, indicating the possibility that mRNAs with different 3′-UTR lengths may coexist. Deduced amino acid sequence of mouse Oatp-5 protein contains 670 amino acids and has 10 putative transmembrane domains, multiple potential glycosylation and phosphorylation sites. Tissue-specific expression studies indicate that mouse Oatp-5 is expressed only in kidney. Studies on the developmental expression reveal that there is no significant expression of Oatp-5 mRNA in mouse kidney for at least 3 weeks after birth, and adult levels of Oatp-5 mRNA expression are attained more than 6 weeks after birth. Phylogenetic analysis reveals that mouse Oatp-5 is an ortholog of rat Oatp-5.