Specific, sensitive, and quantitative enzyme-linked immunosorbent assay for human immunoglobulin G antibodies to anthrax toxin protective antigen.
Specific, sensitive, and quantitative enzyme-linked immunosorbent assay for human immunoglobulin G antibodies to anthrax toxin protective antigen.
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DOI：
10.3201/eid0810.020380
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发表时间：
2002-10
影响因子：
11.8
通讯作者：
Perkins BA
中科院分区：
文献类型：
--
作者：
Quinn CP;Semenova VA;Elie CM;Romero-Steiner S;Greene C;Li H;Stamey K;Steward-Clark E;Schmidt DS;Mothershed E;Pruckler J;Schwartz S;Benson RF;Helsel LO;Holder PF;Johnson SE;Kellum M;Messmer T;Thacker WL;Besser L;Plikaytis BD;Taylor TH Jr;Freeman AE;Wallace KJ;Dull P;Sejvar J;Bruce E;Moreno R;Schuchat A;Lingappa JR;Martin SK;Walls J;Bronsdon M;Carlone GM;Bajani-Ari M;Ashford DA;Stephens DS;Perkins BA
The bioterrorism-associated human anthrax epidemic in the fall of 2001 highlighted the need for a sensitive, reproducible, and specific laboratory test for the confirmatory diagnosis of human anthrax. The Centers for Disease Control and Prevention developed, optimized, and rapidly qualified an enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G (IgG) antibodies to Bacillus anthracis protective antigen (PA) in human serum. The qualified ELISA had a minimum detection limit of 0.06 µg/mL, a reliable lower limit of detection of 0.09 µg/mL, and a lower limit of quantification in undiluted serum specimens of 3.0 µg/mL anti-PA IgG. The diagnostic sensitivity of the assay was 97.8%, and the diagnostic specificity was 94.2%. A competitive inhibition anti-PA IgG ELISA was also developed to enhance diagnostic specificity to 100%. The anti-PA ELISAs proved valuable for the confirmation of cases of cutaneous and inhalational anthrax and evaluation of patients in whom the diagnosis of anthrax was being considered.
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影响因子：
9.4
作者：
PLIKAYTIS, BD;TURNER, SH;CARLONE, GM
通讯作者：
CARLONE, GM
影响因子：
3.2
作者：
STRANGE, RE;THORNE, CB
通讯作者：
THORNE, CB
DOI：
10.20506/rst.17.2.1119
发表时间：
1998-08-01
影响因子：
0.6
作者：
Jacobson, RH
通讯作者：
Jacobson, RH
影响因子：
6.4
作者：
HARRISON, LH;EZZELL, JW;KAUFMANN, AF
通讯作者：
KAUFMANN, AF
DOI：
10.1007/bf01591362
发表时间：
1996-03-01
影响因子：
4.5
作者：
Shlyakhov, E;Rubinstein, E
通讯作者：
Rubinstein, E