The expression of Transmembrane Protein 100 is regulated by alterations in calcium signaling rather than endoplasmic reticulum stress
The expression of Transmembrane Protein 100 is regulated by alterations in calcium signaling rather than endoplasmic reticulum stress
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DOI：
10.1080/09168451.2018.1464899
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发表时间：
2018-01-01
影响因子：
1.6
通讯作者：
Sato, Ryuichiro
中科院分区：
文献类型：
--
作者：
Kuboyama, Ayane;Sasaki, Takashi;Sato, Ryuichiro
跨膜蛋白100（Transmembrane protein 100，TMEM 100）由134个氨基酸残基组成，在脊椎动物中高度保守。Tmem100最近被报道为血管生成、疼痛传递和肿瘤抑制的关键因子。虽然TMEM100功能的重要性得到了很好的支持，但很少有研究阐明其表达机制。在目前的研究中，我们发现，激活转录因子6，内质网（ER）应激激活的转录因子，增强Tmem100启动子活性。在小鼠Tmem100启动子区域中鉴定出两个ER应激反应元件样基序。然而，使用另一种类型的ER应激诱导剂的额外实验证明，在TMEM 100表达的调节中，钙信号传导比ER应激更重要。细胞内钙信号传导控制生物过程，如细胞增殖和胚胎发育。这项研究表明，TMEM100除了响应ER应激外，还响应钙信号传导的改变执行各种功能。
Transmembrane protein 100 (TMEM100) comprises 134 amino acid residues and is highly conserved among vertebrates. Tmem100 has been recently reported as a key factor in angiogenesis, pain transmission, and tumor suppression. Although the importance of TMEM100 function is well supported, few studies have elucidated its expression mechanism. In the current study, we found that activating transcription factor 6, a transcription factor activated by endoplasmic reticulum (ER) stress, enhanced Tmem100 promoter activity. Two ER stress response element-like motifs were identified in the mouse Tmem100 promoter region. However, additional experiments using another type of ER stress inducer demonstrated that calcium signaling was more important than ER stress in the regulation of TMEM100 expression. Intracellular calcium signaling controls biological processes such as cell proliferation and embryonic development. This study suggested that TMEM100 performs various functions in response to alterations in calcium signaling in addition to those in response to ER stress.