SATURATION TRANSFER IN LIVING SYSTEMS
SATURATION TRANSFER IN LIVING SYSTEMS
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DOI：
10.1098/rstb.1980.0061
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
BROWN, TR
中科院分区：
文献类型：
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作者：
BROWN, TR
N.m.r.除了提供关于代谢物水平及其时间依赖性的信息外，对生命系统的研究还可用于获得体内的动力学速率。这通过使用磁化转移技术是可能的，该技术依赖于核自旋将保持在给定量子态达其自旋晶格弛豫时间T1量级的周期的事实，从而使得能够扰动一个分子种类中的核自旋，然后观察该扰动到另一种类的转移，条件是转移发生在T1量级的时间内。在大多数系统中，这些时间约为1 s，因此允许通过该方法测量0.1-10- 1 s范围内的速率。这些技术是由Forsen和霍夫曼在一系列论文中介绍的，这些论文探索了它们在相对简单的化学体系中的应用（Forsen和霍夫曼1963，1964;霍夫曼和Forsen 1969）。早期的生物学应用是Gupta和Redfield（1970）对细胞色素c的应用。该技术已应用于大肠杆菌，获得ATP合成速率（Brownet al.1977），并应用于青蛙肌肉和灌注的大鼠心脏，获得ATP和磷酸肌酸（PCr）的交换速率，该反应在这些系统中由肌酸磷酸激酶（CPK）催化（Brownet al.1978）。
N.m.r. studies of living systems can be used to obtain kinetic ratesin vivo, in addition to providing information about metabolite levels and their time dependences. This is possible through the use of magnetization transfer techniques, which rely on the fact that a nuclear spin will remain in a given quantum state for a period of the order of its spin lattice relaxation time, T1, thus enabling perturbation of the nuclear spins in one molecular species and then observation of the transfer of that perturbation to another species, provided that the transfer takes place in a time of the order of T1. These times are about 1s in most systems, thus allowing the measurement of rates in the range of 0.1-10-1s by this method. These techniques were introduced by Forsen & Hoffman in a series of papers exploring their use in relatively simple chemical systems (Forsen & Hoffman 1963, 1964; Hoffman & Forsen 1969). An early biological application was to cytochrome c, by Gupta & Redfield (1970). The techniques have been applied toEscherichia colito obtain an ATP synthesis rate (Brownet al. 1977) and to frog muscles and perfused rat hearts to obtain the exchange rates of ATP and phosphocreatine (PCr), this reaction being catalysed by creatine phosphokinase (CPK) in these systems (Brownet al. 1978).