2A peptide from ERBV-1 efficiently separates endogenous protein domains in the fission yeast Schizosaccharomyces pombe.
2A peptide from ERBV-1 efficiently separates endogenous protein domains in the fission yeast Schizosaccharomyces pombe.
复制标题
DOI：
10.17912/micropub.biology.000941
复制
发表时间：
2023
影响因子：
--
通讯作者：
Berro, Julien
中科院分区：
文献类型：
--
作者：
Ren, Yuan;Lin, Qun;Berro, Julien
2A肽广泛用于载体上的多顺反子基因表达。相比之下，通过2A肽分离内源基因在很大程度上尚未被探索。我们发现，在分裂酵母裂糖酵母（Schizosaccharomyces pombe）中，来自ERBV-1（马鼻炎B病毒1）的2A肽在不同插入位点对End4的“切割”效率在~70% ~ ~99%之间。我们的研究结果表明，在裂变酵母中使用2A肽分离内源性蛋白结构域具有很高的“切割”效率和相当大的变异。建议验证2A肽的“切割”效率。
2A peptides are widely used for polycistronic gene expression from vectors. In contrast, the separation of endogenous genes via 2A peptides has been largely unexplored. We show that in fission yeast Schizosaccharomyces pombe , the “cleaving” efficiency of the 2A peptide from ERBV-1 (Equine rhinitis B virus 1) range from ~70% to ~99% for End4 at different insertion sites. Our results suggest a high “cleaving” efficiency as well as considerable variation for using 2A peptide to separate endogenous protein domains in fission yeast. Verification of the “cleaving” efficiency of 2A peptides is advised for its application.