PROMOTION AND INHIBITION OF CALCIUM-CARBONATE CRYSTALLIZATION INVITRO BY MATRIX PROTEIN FROM BLUE-CRAB EXOSKELETON
PROMOTION AND INHIBITION OF CALCIUM-CARBONATE CRYSTALLIZATION INVITRO BY MATRIX PROTEIN FROM BLUE-CRAB EXOSKELETON
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DOI：
10.2307/1541769
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发表时间：
1990-10-01
影响因子：
1.6
通讯作者：
WHEELER, AP
中科院分区：
文献类型：
--
作者：
GUNTHORPE, ME;SIKES, CS;WHEELER, AP
可溶性有机基质分离的背甲的青蟹，Callinectes sapidus，抑制碳酸钙结晶时，在溶液中自由。固定化基质复合物，通过交联可溶性基质脱钙蟹甲壳制备，促进碳酸钙的形成，在固定化可溶性基质复合物的存在下，结晶开始早于在溶液中的控制。在实验处理中，晶体的沉积只发生在络合物中，而不是在结晶溶液中。甲壳素，N-乙酰基-D-葡糖胺的聚合物，和壳聚糖，脱乙酰甲壳素，这两者都是不溶性产品的有机基质的蟹甲壳含有很少或没有基质蛋白质，没有促进碳酸钙结晶。壳聚糖固定化聚阴离子合成肽复合物也促进了碳酸钙结晶。添加一个疏水性的尾巴（Ala 8）的聚阴离子肽（Asp 20）的促进率降低，可能是因为疏水性的尾巴形成了一个扩散屏障周围的晶核生长位点，抑制新生的晶核与离子在本体溶液中的相互作用。
Soluble organic matrix isolated from dorsal carapaces of the blue crab, Callinectes sapidus, inhibited CaCO3 crystallization when free in solution. Immobilized matrix complexes, prepared by crosslinking soluble matrix to decalcified crab carapace, promoted CaCO3 formation in that crystallization in the presence of the immobilized soluble matrix complexes began sooner than in solution controls. In the experimental treatments, deposition of crystals occurred only within the complexes and not in the crystallization solutions. Chitin, a polymer of N-acetyl-D-glucosamine, and chitosan, a deacetylated chitin, which are both insoluble products of the organic matrix of the crab carapace containing little to no matrix protein, did not promote CaCO3 crystallization. Complexes of immobilized polyanionic synthetic peptides on chitosan also promoted CaCO3 crystallization. Addition of a hydrophobic tail (Ala8) to the polyanionic peptide (Asp20) reduced the rate of promotion, possibly because the hydrophobic tail formed a diffusion barrier around crystal nuclei growth sites, suppressing interactions of nascent crystal nuclei with ions in the bulk solution.