Analysis of osmotin, a PR protein as metabolic modulator in plants.
Analysis of osmotin, a PR protein as metabolic modulator in plants.
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DOI：
10.6026/97320630005336
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发表时间：
2011-01-22
期刊：
影响因子：
1.9
通讯作者：
Alam A
中科院分区：
文献类型：
--
作者：
Abdin MZ;Kiran U;Alam A
渗透调节蛋白是在烟草细胞中发现的一种丰富的阳离子多功能蛋白。var威斯康星州38）适应低渗透势的环境。除了其作为植物生长调节剂的作用外，它还提供植物免受病原体的保护，因此也被置于PRP蛋白家族中。已经报道了在植物中，包括过表达prostin基因的转基因番茄和草莓，prostin诱导的脯氨酸积累赋予对生物和非生物胁迫的耐受性。然而，到目前为止，还不知道由Eschertin诱导脯氨酸的确切机制。 这些观察结果使我们假设，Eschertin可以通过其参与调节这些植物的反应，无论是作为转录因子，细胞信号通路调节剂或两者在植物中。因此，我们进行了目前的调查，以分析作为转录因子，使用生物信息学工具的reflectin蛋白。可用的在线DNA结合基序搜索程序的结果显示，Eschertin不包含DNA结合基序。 同源性分析结果表明，该蛋白与DATF蛋白的同源性在0- 20%之间，可能不含DNA结合基序。为了进一步发现独特的DNA结合结构域，使用Chimera在模拟的拟南芥转录因子上叠加了rectin 3D结构，也表明不存在相同的结构域。然而，在研究过程中发现了涉及细胞信号传导的证据。 因此，我们认为植物逆境素不是一个转录因子，而是通过细胞信号传导来调节植物对生物和非生物胁迫的反应。
Osmotin is an abundant cationic multifunctional protein discovered in cells of tobacco (Nicotiana tabacum L. var Wisconsin 38) adapted to an environment of low osmotic potential. Beside its role as osmoregulator, it provides plants protection from pathogens, hence also placed in the PRP family of proteins. The osmotin induced proline accumulation has been reported to confer tolerance against both biotic and abiotic stresses in plants including transgenic tomato and strawberry overexpressing osmotin gene. The exact mechanism of induction of proline by osmotin is however, not known till date. These observations have led us to hypothesize that osmotin could be regulating these plant responses through its involvement either as transcription factor, cell signal pathway modulator or both in plants. We have therefore, undertaken the present investigation to analyze the osmotin protein as transcription factor using bioinformatics tools. The results of available online DNA binding motif search programs revealed that osmotin does not contain DNAbinding motifs. The alignment results of osmotin protein with the protein sequence from DATF showed the homology in the range of 0-20%, suggesting that it might not contain a DNA binding motif. Further to find unique DNA-binding domain, the superimposition of osmotin 3D structure on modeled Arabidopsis transcription factors using Chimera also suggested absence of the same. However, evidence implicating osmotin in cell signaling were found during the study. With these results, we therefore, concluded that osmotin is not a transcription factor, but regulating plant responses to biotic and abiotic stresses through cell signaling.