Proteomic analysis of rat brain related to mass effect after experimental intracerebral hemorrhage
Proteomic analysis of rat brain related to mass effect after experimental intracerebral hemorrhage
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DOI：
10.1016/j.hest.2021.01.001
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发表时间：
2021-02
期刊：
影响因子：
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通讯作者：
Yumei Wang;Zongkun Hou;Yuhua Gong;Shilei Hao;Bochu Wang
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文献类型：
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作者：
Yumei Wang;Zongkun Hou;Yuhua Gong;Shilei Hao;Bochu Wang
相对于脑出血后继发性脑损伤，占位效应引起的原发性脑损伤机制的报道较少。本文采用串联质量标签（TMT）标记蛋白质组学方法研究脑出血后质量效应的机械损伤。共鉴定了5390个蛋白质，定量了4593个蛋白质，其中80个蛋白质表达上调（M/N比值> 1.2），30个蛋白质表达下调（M/N比值> 1.2）。生物信息学和富集分析表明，差异表达蛋白主要参与免疫应答、信号传递和氧化磷酸化。质量效应通过机械拉伸和挤压脑组织来影响ATP的合成和代谢。此外，质量效应还显著影响神经元的细胞形态和树突发育，并通过形成氧化应激和氧糖剥夺等机制引起神经元损伤，激活神经元的保护和防御机制。本研究结果提供了脑出血后占位效应致原发性脑损伤的相关通路和关键蛋白，为进一步认识脑出血损伤机制提供了科学依据。
Compared to the secondary brain injury after intracerebral haemorrhage (ICH), fewer reports focused on the mechanism of primary brain injury induced by mass effect. Here, the tandem mass tag (TMT) labeling proteomics method was used to study the mechanical damage of the mass effect after ICH. A total of 5390 proteins were identified, and 4593 proteins were quantified, included 80 up-regulated proteins (M/N Ratio > 1.2) and 30 down-regulated proteins (M/N Ratio > 1.2). Bioinformatics and enrichment analysis showed that the differentially expressed proteins were mainly involved in immune response, signal transmission, and oxidative phosphorylation. The mass effect affects the synthesis and metabolism of ATP by mechanically stretching and squeezing the brain tissue. Furthermore, the cell morphology and dendritic development of neurons were also significantly influenced by mass effect, which also cause neuronal damage through the formation of oxidative stress and oxygen-glucose deprivation, and activate the neuronal protection and defense mechanisms. Our results provide the relevant channels and key proteins of primary brain injury caused by mass effect after ICH, which offer a scientific basis for the understanding of ICH injury.