Preliminary study of a challenge test to the patients with Japanese cedar pollinosis using an environmental exposure unit
Preliminary study of a challenge test to the patients with Japanese cedar pollinosis using an environmental exposure unit
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DOI：
10.1016/j.anl.2010.04.001
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发表时间：
2010-12-01
影响因子：
1.7
通讯作者：
Ro, Toshitami
中科院分区：
文献类型：
--
作者：
Ito, Kanako;Terada, Tetsuya;Ro, Toshitami
目的：虽然对花粉症的临床研究已经进行了安慰剂对照双盲比较和现场技术，准确的评价是困难的，因为散射花粉计数和气候条件不同的年份和地区，导致结果的偏差。因此，我们准备了一个花粉挑战测试单元（过敏原挑战室：ACC），便于定量花粉挑战在任何时候，所以，收购客观data.Methods：恒定条件的控制和维护指定的花粉浓度在ACC进行了研究。此外，在保持10，000计数/m3的水平的同时，测量了ACC中的花粉分布。采用抽吸法和达勒姆法测量花粉水平，并比较测量值。结果：当ACC中的花粉水平设置为6000计数/m3或更高时，变化率为+/-15%，在可调范围内，花粉在室内的分布在+/-20%以内。结论：ACC中花粉水平的时空变化较小，有利于花粉激发的稳定性，并可诱发花粉症症状。激发室可用于判断花粉症治疗的效果。(C)2010爱思唯尔爱尔兰有限公司版权所有。
Objective: Although clinical studies on pollinosis have been performed employing placebo-controlled double-blind comparative and field techniques, accurate evaluation is difficult because the scattering pollen count and climatic conditions vary between years and regions, leading to a bias in the results. Thus, we prepared a pollen challenge test unit (allergen challenge chamber: ACC) which facilitates quantitative pollen challenge at any time, and, so, the acquisition of objective data.Methods: The control of constant conditions and maintenance of specified pollen concentrations in ACC were investigated. In addition, the pollen distribution in ACC was measured while maintaining the level at 10,000 counts/m(3). The pollen levels were measured employing the aspiration and Durham methods, and the measured values were compared. Furthermore, whether symptoms are adequately induced in the chamber during the non-cedar pollen-scattering season was investigated in 14 volunteers with cedar pollinosis.Results: When the pollen level in ACC was set at 6000 counts/m(3) or higher, the rate of variation was +/-15%, within the adjustable range, and that of the pollen distribution in the chamber was within +/-20%. When the volunteers with cedar pollinosis were exposed to cedar pollen in ACC, pollinosis symptoms were induced, and challenge for 2 consecutive days significantly induced symptoms.Conclusions: The temporal and spatial variations of the pollen level in ACC were small, facilitating stable pollen challenge, and pollinosis symptoms were induced in the volunteers with cedar pollinosis. The challenge chamber may be useful to judge the effects of therapy against pollinosis. (C) 2010 Elsevier Ireland Ltd. All rights reserved.